Biogas is expected to make an important contribution to the vision of fossil-free transports in Sweden. However, estimates of the national production potential have taken a top-down perspective, without detailing where the potential exists and how to realise it. This study is made with a bottom-up perspective, investigating the potential for production and use of biogas within different sectors and individual industries in the municipality of Norrköping. Moreover, critical factors and driving actors for realising these potentials are raised and analysed. The study was conducted with a participatory approach involving 22 representatives from the municipality, biogas producers, interest organisations and companies dealing with potential biogas substrates. The results indicate a potential biogas production of 500 GWh/year by 2030, out of which 60% would come from the agricultural sector and 30% from local pulp and paper industries. A more modest estimate indicate that the production would cover 10 – 15% of the local energy demand for road transport and shipping as well as industrial energy gas. Substrates are distributed over a large geographical area and between several actors, requiring cooperation between substrate owners to reach an economically feasible scale. In addition, collaboration with biogas companies could provide the substrate owners with necessary specialist knowledge. In order to realise the biogas potential, Norrköping municipality has a central role to play as coordinator and knowledge hub, as well as by directing procurements towards biogas and plan for biogas fuelling stations.