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Assessment of the transfer, persistence, prevalence and recovery of DNA traces from clothing: An inter-laboratory study on worn upper garments
Deakin Univ, Australia; Victoria Police Forens Serv Dept, Australia.
Linköping University, Department of Physics, Chemistry and Biology, Biology. Linköping University, Faculty of Science & Engineering. Swedish Natl Forens Ctr, Linkoping, Sweden.
Swedish Natl Forens Ctr, Linkoping, Sweden.
Forens Sci Ireland, Ireland.
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2019 (English)In: Forensic Science International: Genetics, ISSN 1872-4973, E-ISSN 1878-0326, Vol. 42, p. 56-68Article in journal (Refereed) Published
Abstract [en]

Among the various items recovered from crime scenes or persons involved in a crime event, clothing items are commonly encountered and submitted for forensic DNA sampling. Depending on the case circumstances and the activity-of-interest, sampling of the garment may concentrate on collecting DNA from the wearer, or from one or more offenders who have allegedly contacted the item and/or wearer. Relative to the targeted DNA, background DNA already residing on the item from previous contacts, or transferred during or after the crime event, may also be collected during sampling and observed in the resultant DNA profile. Given our limited understanding of how, and from where, background DNA is derived on clothing, research on the transfer, persistence, prevalence, and recovery (TPPR) of DNA traces from upper garments was conducted by four laboratories. Samples were collected from several areas of two garments, each worn on separate working or non-working days and individually owned by four individuals from each of the four laboratories, and processed from DNA extraction through to profiling. Questionnaires documented activities relating to the garment prior to and during wearing, and reference profiles were obtained from the wearer and their close associates identified in the questionnaire. Among the 448 profiles generated, variation in the DNA quantity, composition of the profiles, and inclusion/exclusion of the wearer and their close associates was observed among the collaborating laboratories, participants, garments worn on different occasions, and garment areas sampled.

Place, publisher, year, edition, pages
ELSEVIER IRELAND LTD , 2019. Vol. 42, p. 56-68
Keywords [en]
DNA; Transfer; Persistence; Prevalence; Recovery; Clothing; Activity level; Inter-laboratory study
National Category
Genetics
Identifiers
URN: urn:nbn:se:liu:diva-160395DOI: 10.1016/j.fsigen.2019.06.011ISI: 000483955000014PubMedID: 31229887OAI: oai:DiVA.org:liu-160395DiVA, id: diva2:1353567
Note

Funding Agencies|Prevention of and Fight against Crime (ISEC) programme of the European Union [HOME/2013/ISEC/MO/ENFSI/4000005962]; European Commission

Available from: 2019-09-23 Created: 2019-09-23 Last updated: 2019-09-23

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Citation style
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