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Application value of CyTOF 2 mass cytometer technology at single-cell level in human gastric cancer cells
Hebei Med Univ, Peoples R China.
Hebei Med Univ, Peoples R China.
Linköping University, Department of Clinical and Experimental Medicine, Division of Surgery, Orthopedics and Oncology. Linköping University, Faculty of Medicine and Health Sciences. Region Östergötland, Center for Surgery, Orthopaedics and Cancer Treatment, Department of Oncology.
Orebro Univ, Sweden.
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2019 (English)In: Experimental Cell Research, ISSN 0014-4827, E-ISSN 1090-2422, Vol. 384, no 1, article id UNSP 111568Article in journal (Refereed) Published
Abstract [en]

Chemotherapy and radiotherapy are main adjuvant therapies for the treatment of gastric cancer, the treatment effects are individual difference, but the specific mechanism is unknown. CyTOF 2 mass cytometer (CyTOF) enables the detecting up to 135 parameters on single cell, the emergence of which is an opportunity for proteomics research. We first tried to apply CyTOF technique to gastric cancer cells. We verified applicability of CyTOF in gastric cancer cells, and analyzed the responses of seventeen proteins to chemoradiotherapy in human gastric cancer AGS cells. To analyze the high dimensional CyTOF data, we used two statistical and visualization tools including viSNE and Citrus. Two specific clusters were found which had differences in protein expression profiles. CyTOF technology is proved feasibility and value at single cell level of gastric cancer.

Place, publisher, year, edition, pages
ELSEVIER INC , 2019. Vol. 384, no 1, article id UNSP 111568
Keywords [en]
Mass cytometry; Gastric cancer; Single-cell; viSNE; Citrus
National Category
Cancer and Oncology
Identifiers
URN: urn:nbn:se:liu:diva-161373DOI: 10.1016/j.yexcr.2019.111568ISI: 000490031700024PubMedID: 31446162OAI: oai:DiVA.org:liu-161373DiVA, id: diva2:1367568
Note

Funding Agencies|Hebei Province Key Research and Development Project [18277741D]; International Science & Technology Cooperation Program of China [2014DFA31150]; Hebei Province Projects [1387, SGH201501, A201802017, LNB201809, G2019035]

Available from: 2019-11-04 Created: 2019-11-04 Last updated: 2019-11-04

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Sun, Xiao-Feng
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Division of Surgery, Orthopedics and OncologyFaculty of Medicine and Health SciencesDepartment of Oncology
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