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Induction of CIRBP expression by cold shock on bovine cumulus-oocyte complexes
Univ Autonoma Barcelona, Spain.
Univ Autonoma Barcelona, Spain.
Linköping University, Department of Clinical and Experimental Medicine, Division of Children's and Women's health. Linköping University, Faculty of Medicine and Health Sciences. Univ Autonoma Barcelona, Spain.
Univ Autonoma Barcelona, Spain.
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2019 (English)In: Reproduction in domestic animals, ISSN 0936-6768, E-ISSN 1439-0531, Vol. 54, p. 82-85Article in journal (Refereed) Published
Abstract [en]

The aim of this study was to induce the cold-inducible RNA-binding protein (CIRBP) expression on cumulus-oocyte complexes (COCs) through exposure to a sub-lethal cold shock and determine the effects of hypothermic temperatures during the in vitro maturation of bovine oocytes. Nuclear maturation, cortical granule redistribution and identification of cold-inducible RNA-binding protein (CIRBP) were assessed after 24 hr of in vitro maturation of control (38.5 degrees C) and cold-stressed oocytes (33.5 degrees C). The presence of CIRBP was assessed by Western blot in COCs or denuded oocytes and their respective cumulus cells. Based on the odds ratio, cold-stressed oocytes presented higher abnormal cytoplasmic distribution of cortical granules and nuclear maturation than the control group. Although CIRBP was detected in both control and cold-stressed groups, cold-stressed COCs had 2.17 times more expression of CIRBP than control COCs. However, when denuded oocytes and cumulus cells were assessed separately, CIRBP only was detected in cumulus cells in both groups. In conclusion, cold shock induced CIRBP expression, but it negatively affected nuclear maturation and cortical granule distribution of bovine oocytes. Moreover, the expression of CIRBP was only identified in cumulus cells but not in oocytes.

Place, publisher, year, edition, pages
WILEY , 2019. Vol. 54, p. 82-85
Keywords [en]
CIRBP protein; cold-shock response; domestic cow; in vitro oocyte maturation
National Category
Cell Biology
Identifiers
URN: urn:nbn:se:liu:diva-161620DOI: 10.1111/rda.13518ISI: 000491448500015PubMedID: 31625234OAI: oai:DiVA.org:liu-161620DiVA, id: diva2:1367905
Note

Funding Agencies|FI grant [2018 FI_B 00236]; [AGL2016-79802-P]; [AGL2016-81890-REDT]; [FPU2015/06029]; [IJCI-2015-24380]

Available from: 2019-11-05 Created: 2019-11-05 Last updated: 2019-11-05

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Alvarez-Rodriguez, Manuel
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Division of Children's and Women's healthFaculty of Medicine and Health Sciences
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