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Supplementation with exogenous coenzyme Q10 to media for in vitro maturation and embryo culture fails to promote the developmental competence of porcine embryos
Univ Murcia, Spain; SaBio IREC CSIC UCLM JCCM, Spain.
Linköping University, Department of Clinical and Experimental Medicine, Division of Children's and Women's health. Linköping University, Faculty of Medicine and Health Sciences. Univ Murcia, Spain.
Univ Murcia, Spain.
Univ Murcia, Spain.
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2019 (English)In: Reproduction in domestic animals, ISSN 0936-6768, E-ISSN 1439-0531, Vol. 54, p. 72-77Article in journal (Refereed) Published
Abstract [en]

The coenzyme Q10 (CoQ10) is a potent antioxidant with critical protection role against cell oxidative stress, caused by the mitochondrial dysfunction. This study evaluated the effects of CoQ10 supplementation to in vitro maturation (IVM) or embryo culture media on the maturation, fertilization and subsequent embryonic development of pig oocytes and embryos. Maturation (Experiment 1) or embryo culture (Experiment 2) media were supplemented with 0 (control), 10, 25, 50 and 100 mu M CoQ10. The addition of 10-50 mu M CoQ10 to the IVM medium did not affect the percentage of MII oocytes nor the fertilization or the parameters of subsequent embryonic development. Exogenous CoQ10 in the culture medium neither did affect the development to the 2-4-cell stage nor rates of blastocyst formation. Moreover, the highest concentration of CoQ10 (100 mu M) in the maturation medium negatively affected blastocyst rates. In conclusion, exogenous CoQ10 supplementation of maturation or embryo culture media failed to improve the outcomes of our in vitro embryo production system and its use as an exogenous antioxidant should not be encouraged.

Place, publisher, year, edition, pages
WILEY , 2019. Vol. 54, p. 72-77
Keywords [en]
antioxidant; coenzyme Q10; embryo culture medium; maturation medium; porcine
National Category
Developmental Biology
Identifiers
URN: urn:nbn:se:liu:diva-161619DOI: 10.1111/rda.13486ISI: 000491448500013PubMedID: 31625244OAI: oai:DiVA.org:liu-161619DiVA, id: diva2:1367906
Note

Funding Agencies|Seneca Foundation, Murcia, SpainFundacion Seneca [20027/SF/16, 19892/GERM/15]; Junta de Comunidades de Castilla-La Mancha, SpainJunta de Castilla y Leon [SBPLY/17/180501/000500]; Ministry of Economy and Competitiveness [BES-2013-064087, BES-2016-077869]; MINECO-FEDER [AGL2015-69735-R]; Research Council FORMAS, Stockholm [2017-00946]

Available from: 2019-11-05 Created: 2019-11-05 Last updated: 2019-11-05

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