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Comparative evaluation of DNA integrity using sperm chromatin structure assay and Sperm-Ovis-Halomax during in vitro capacitation of cryopreserved ram spermatozoa
SaBio IREC CSIC UCLM JCCM, Spain.
Linköping University, Department of Clinical and Experimental Medicine, Division of Children's and Women's health. Linköping University, Faculty of Medicine and Health Sciences.
SaBio IREC CSIC UCLM JCCM, Spain.
SaBio IREC CSIC UCLM JCCM, Spain.
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2019 (English)In: Reproduction in domestic animals, ISSN 0936-6768, E-ISSN 1439-0531, Vol. 54, p. 46-49Article in journal (Refereed) Published
Abstract [en]

This study aimed to compare the ability of sperm chromatin structure assay (SCSA (R)) and Sperm-Ovis-Halomax (R) to detect DNA fragmentation in frozen-thawed ram spermatozoa incubated under capacitating conditions in synthetic oviductal fluid (SOF) supplemented with oestrous sheep serum (SOF-ESS) at multiple time points (0-240 min). Incubation in SOF-ESS had no significant effects on SCSA (R) parameters while the percentage of spermatozoa with fragmented DNA measured by Sperm-Ovis-Halomax (R) increased after 180 min of incubation. In addition, no correlation or agreement was found between the techniques, suggesting that SCSA (R) and Sperm-Ovis-Halomax (R) may quantify different types of DNA damage in ram spermatozoa under these experimental conditions.

Place, publisher, year, edition, pages
WILEY , 2019. Vol. 54, p. 46-49
Keywords [en]
DNA integrity; sperm capacitation; SCSA (R); Sperm-Ovis-Halomax (R)
National Category
Biophysics
Identifiers
URN: urn:nbn:se:liu:diva-161618DOI: 10.1111/rda.13519ISI: 000491448500007PubMedID: 31625230OAI: oai:DiVA.org:liu-161618DiVA, id: diva2:1367907
Note

Funding Agencies|ALF-Research, Region Ostergotland [LIO-698951]; Lions Forskningsfond [DNR LIU-2016-00641]; Linkoping and the Swedish Research Council FormasSwedish Research Council Formas [2017-00946]; Forskningsradet i Sydostra Sverige [473121, 745971]; Universidad de Castilla-La Mancha [2015/4062]

Available from: 2019-11-05 Created: 2019-11-05 Last updated: 2019-11-05

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Alvarez-Rodriguez, ManuelRodriguez-Martinez, Heriberto
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Division of Children's and Women's healthFaculty of Medicine and Health Sciences
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