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Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition)
Univ Modena and Reggio Emilia, Italy.
Deutsch Rheuma Forschungszentrum DRFZ, Germany.
Deutsch Rheuma Forschungszentrum DRFZ, Germany.
Friedrich Alexander Univ Erlangen Nuremberg, Germany.
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2019 (English)In: European Journal of Immunology, ISSN 0014-2980, E-ISSN 1521-4141, Vol. 49, no 10, p. 1457-1973Article in journal (Refereed) Published
Abstract [en]

These guidelines are a consensus work of a considerable number of members of the immunology and flow cytometry community. They provide the theory and key practical aspects of flow cytometry enabling immunologists to avoid the common errors that often undermine immunological data. Notably, there are comprehensive sections of all major immune cell types with helpful Tables detailing phenotypes in murine and human cells. The latest flow cytometry techniques and applications are also described, featuring examples of the data that can be generated and, importantly, how the data can be analysed. Furthermore, there are sections detailing tips, tricks and pitfalls to avoid, all written and peer-reviewed by leading experts in the field, making this an essential research companion.
Place, publisher, year, edition, pages
WILEY , 2019. Vol. 49, no 10, p. 1457-1973
National Category
Immunology
Identifiers
URN: urn:nbn:se:liu:diva-162559DOI: 10.1002/eji.201970107ISI: 000491166500003PubMedID: 31633216OAI: oai:DiVA.org:liu-162559DiVA, id: diva2:1379037
Note

Funding Agencies|Deutsche Forschungsgemeinschaft (DFG, German Research Foundation)German Research Foundation (DFG) [125440785 - SFB 877]; Bavarian Immune Therapy Network (BayImmuNet); Netherlands Organisation for Scientific Research - Domain Applied and Engineering Sciences (NWO-TTW), research program VENI [15924]; DFGGerman Research Foundation (DFG) [MA 2273/14-1]; Excellence Cluster "Inflammation at Interfaces" [EXC 306/2]; German Research FoundationGerman Research Foundation (DFG) [ME 3644/5-1, TRR130 TP24]; NIHUnited States Department of Health & Human ServicesNational Institutes of Health (NIH) - USA [R50CA211108]; Deutsche ForschungsgemeinschaftGerman Research Foundation (DFG) [TRR130, C03]; Germany Research FoundationGerman Research Foundation (DFG) [CRC738/B3]; German Rheumatism Research Centre Berlin; European Union Innovative Medicines Initiative - Joint Undertaking [777357]; Else Kroner-Fresenius-Foundation, German Federal Ministry of Education and Research e:Med sysINFLAME Program [01ZX1306B]; KMU-innovativ "InnoCyt"; Leibniz Science Campus; ISAC SRL Emerging Leaders program; European Research Council under the European UnionEuropean Research Council (ERC) [695551]

Available from: 2019-12-16 Created: 2019-12-16 Last updated: 2022-09-30

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