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In vitro measurement of glucose uptake after radiation and cetuximab treatment in head and neck cancer cell lines using 18F-FDG, gamma spectrometry and PET/CT
Linköping University, Department of Clinical and Experimental Medicine, Division of Cell Biology. Linköping University, Faculty of Medicine and Health Sciences.
Linköping University, Department of Biomedical Engineering. Linköping University, Faculty of Science & Engineering.
Linköping University, Department of Clinical and Experimental Medicine, Division of Cell Biology. Linköping University, Faculty of Medicine and Health Sciences.
Linköping University, Department of Clinical and Experimental Medicine, Division of Cell Biology. Linköping University, Faculty of Medicine and Health Sciences. Region Östergötland, Anaesthetics, Operations and Specialty Surgery Center, Department of Otorhinolaryngology.
2019 (English)In: Oncology Letters, ISSN 1792-1074, E-ISSN 1792-1082, Vol. 18, no 5, p. 5155-5162Article in journal (Refereed) Published
Abstract [en]

The standard treatment for head and neck squamous cell carcinoma (HNSCC) is radiotherapy, often in combination with chemotherapy or surgery. However, a novel monoclonal antibody, cetuximab (Erbitux (R)), has also been approved for patient therapy. The aim of present study was to develop an in vitro method for the measurement of 18F-fluoro-2deoxy-D-glucose (FDG) to determine if cellular 18F-FDG uptake is associated with response to radiotherapy or cetuximab treatment. In the current study, HNSCC cell lines were treated with radiation or with cetuximab. Next, the uptake of 18F-FDG was measured using a gamma spectrometer (GS). Thereafter, uptake after radiation was measured first with GS and then compared with positron emission tomography (PET)/computed tomography (CT) imaging. Furthermore, the mRNA expression of glucose transporter 1 (GLUT1) was measured following cetuximab treatment via reverse transcription-quantitative PCR. A study protocol was developed to measure the cellular uptake of 18F-FDG via gamma-ray spectrometry and comparable results were obtained with those of clinical PET/CT. The results revealed a decrease in 18F-FDG after radiation and cetuximab treatment. The uptake of 18F-FDG following cetuximab treatment was significantly lower in the cetuximab-sensitive cell line UT-SCC-14 compared with the cetuximab-resistant cell lines UT-SCC-2 and UT-SCC-45. Furthermore, after treatment with cetuximab for 24 and 48 h, a significant increase in GLUT1 expression was detected in the sensitive cell line compared with the two resistant cell lines. In conclusion, a novel yet reliable method for the measurement of intracellular 18F-FDG via GS has been developed, and our results indicate that 18F-FDG uptake is associated with radiation and cetuximab response in HNSCC.

Place, publisher, year, edition, pages
SPANDIDOS PUBL LTD , 2019. Vol. 18, no 5, p. 5155-5162
Keywords [en]
head and neck squamous cell carcinoma; radiotherapy; cetuximab; metabolism; 18F-fluoro-2deoxy-D-glucose; glucose transporter 1
National Category
Cancer and Oncology
Identifiers
URN: urn:nbn:se:liu:diva-162970DOI: 10.3892/ol.2019.10916ISI: 000503219600083PubMedID: 31620196OAI: oai:DiVA.org:liu-162970DiVA, id: diva2:1382341
Note

Funding Agencies|Swedish Cancer SocietySwedish Cancer Society [2010/545, 2017/301]; ALF Grants; Region Ostergotland; Linkoping University Hospital

Available from: 2020-01-02 Created: 2020-01-02 Last updated: 2020-02-03

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Matic, NatasaRessner, MarcusWiechec, EmiliaRoberg, Karin
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Division of Cell BiologyFaculty of Medicine and Health SciencesDepartment of Biomedical EngineeringFaculty of Science & EngineeringDepartment of Otorhinolaryngology
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Oncology Letters
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