liu.seSearch for publications in DiVA
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • oxford
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Construction of a system for heterologous production of carbonic anhydrase from Plasmodium falciparum in Pichia pastoris
Linköping University, The Department of Physics, Chemistry and Biology. (Molecular Biotechnology Division)
2008 (English)Independent thesis Advanced level (degree of Master), 20 points / 30 hpStudent thesis
Abstract [en]

Malaria is one of the biggest current global health problems, and with the increasing occurance of drug resistant Plasmodium falciparum strains, there is an urgent need for new antimalarial drugs. Given the important role of carbonic anhydrase in Plasmodium falciparum (PfCA), it is a potential novel drug target. Heterologous expression of malaria proteins is problematic due to the unusual codon usage of the Plasmodium genome, so to overcome this problem a synthetic PfCA gene was designed, optimized for expression in Pichia pastoris. This gene was also modified to avoid glycosylation, and cloned into the vector pPICZαA under the control of the methanol inducible promoter AOX1. To facilitate export of the protein into the growth medium, the gene was fused in-frame with the α-factor secretion signal from Saccharomyces cerevisiae. The construct was successfully integrated in the genome of P. pastoris GS115, and attempts were made to express the protein and purify it using immobilized metal ion affinity chromatography.In this work, no expression of the PfCA protein could be detected, so further research should focus on optimization of expression conditions, or redesign of the expression vector.

Place, publisher, year, edition, pages
2008. , 42 p.
Keyword
Carbonic anhydrase, Plasmodium, Pichia pastoris, expression system
National Category
Biochemistry and Molecular Biology
Identifiers
URN: urn:nbn:se:liu:diva-12454ISRN: LITH-IFM-EX-08/2009-SEOAI: oai:DiVA.org:liu-12454DiVA: diva2:139
Uppsok
fysik/kemi/matematik
Supervisors
Examiners
Available from: 2008-09-24 Created: 2008-09-05 Last updated: 2008-09-24

Open Access in DiVA

fulltext(1761 kB)538 downloads
File information
File name FULLTEXT01.pdfFile size 1761 kBChecksum SHA-512
f27532c0264816e7af16077bc9ecc201f826904603b60d6dc9af2bdd54188f95c6ee31634a6e9a4c16c25a81edff823ed75a3ba9b380471e109ec0eee3c5f07d
Type fulltextMimetype application/pdf
attachment(126 kB)89 downloads
File information
File name ATTACHMENT01.pdfFile size 126 kBChecksum SHA-512
0f401113b40050c44eb96f91a50a9dad8874564fcc7308e8eeb9a1bf4e6d21f0af0648d1f5b4257182cf9fe9b0afc9c3a56958167639a5a41a8520b41460a651
Type attachmentMimetype application/pdf

By organisation
The Department of Physics, Chemistry and Biology
Biochemistry and Molecular Biology

Search outside of DiVA

GoogleGoogle Scholar
Total: 538 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

urn-nbn

Altmetric score

urn-nbn
Total: 300 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • oxford
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf