Gambling has been a common activity across societies and cultures. To better understand gambling gender differences in Spain it is important to consider cultural aspects as well as the epidemiology of gambling and pathological gambling in this country. However, it is important to highlight that no national epidemiological studies have yet been conducted in Spain as a whole and the estimated prevalence results from regional studies carried out in Catalonia, Andalusia and Galicia. Few studies have explored gender differences and very few have been conducted so far in Spain. When focusing on gambling behavior during treatment, a recent study describes different gambling patterns between the sexes in a Spanish population. Given the low number of studies exploring female gambling behaviors, the aim of the study was to provide a self-report questionnaire to assess responsible gambling behaviors in Spanish females. As for predictors of gambling behavior, low socioeconomic status emerged as the unique socio-demographic factor for problematic gambling.