A Multi-responsive Fluorescent Probe Reveals Mitochondrial Nucleoprotein Dynamics with Reactive Oxygen Species Regulation through Super-resolution ImagingShow others and affiliations
2020 (English)In: Angewandte Chemie International Edition, ISSN 1433-7851, E-ISSN 1521-3773, Vol. 59, no 37, p. 16154-16160Article in journal (Refereed) Published
Abstract [en]
Understanding the biomolecular interactions in a specific organelle has been a long-standing challenge because it requires super-resolution imaging to resolve the spatial locations and dynamic interactions of multiple biomacromolecules. Two key difficulties are the scarcity of suitable probes for super-resolution nanoscopy and the complications that arise from the use of multiple probes. Herein, we report a quinolinium derivative probe that is selectively enriched in mitochondria and switches on in three different fluorescence modes in response to hydrogen peroxide (H2O2), proteins, and nucleic acids, enabling the visualization of mitochondrial nucleoprotein dynamics. STED nanoscopy reveals that the proteins localize at mitochondrial cristae and largely fuse with nucleic acids to form nucleoproteins, whereas increasing H(2)O(2)level leads to disassociation of nucleic acid-protein complexes.
Place, publisher, year, edition, pages
WILEY-V C H VERLAG GMBH , 2020. Vol. 59, no 37, p. 16154-16160
Keywords [en]
imaging probes; nucleic acids; proteins; reactive oxygen species; super-resolution microscopy
National Category
Biophysics
Identifiers
URN: urn:nbn:se:liu:diva-168539DOI: 10.1002/anie.202005959ISI: 000548161900001PubMedID: 32573047OAI: oai:DiVA.org:liu-168539DiVA, id: diva2:1462170
Note
Funding Agencies|National Natural Science Foundation of ChinaNational Natural Science Foundation of China [21775001, 21705001, 21974001, 21976183]; Anhui Provincial Natural Science Foundation of ChinaNatural Science Foundation of Anhui Province [1808085MB32]; Hefei Physical Science Center Foundation [2017FXZY001]
2020-08-282020-08-282022-10-03