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Colonic paracellular permeability and circulating zonulin-related proteins
Linköping University, Department of Biomedical and Clinical Sciences, Division of Molecular Medicine and Virology. Linköping University, Faculty of Medicine and Health Sciences.
Linköping University, Department of Biomedical and Clinical Sciences, Division of Inflammation and Infection. Linköping University, Faculty of Medicine and Health Sciences. Region Östergötland, Center for Surgery, Orthopaedics and Cancer Treatment, Mag- tarmmedicinska kliniken.
Linköping University, Department of Biomedical and Clinical Sciences, Division of Surgery, Orthopedics and Oncology. Linköping University, Faculty of Medicine and Health Sciences. Region Östergötland, Center for Surgery, Orthopaedics and Cancer Treatment, Mag- tarmmedicinska kliniken.
Linköping University, Department of Biomedical and Clinical Sciences, Division of Surgery, Orthopedics and Oncology. Linköping University, Faculty of Medicine and Health Sciences. Region Östergötland, Center for Surgery, Orthopaedics and Cancer Treatment, Department of Surgery in Linköping.ORCID iD: 0000-0002-3250-5367
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2021 (English)In: Scandinavian Journal of Gastroenterology, ISSN 0036-5521, E-ISSN 1502-7708, Vol. 56, no 4, p. 424-431Article in journal (Refereed) Published
Abstract [en]

Objective Irritable bowel syndrome (IBS) is a gut-brain disorder associated with increased gut permeability. Zonulin has been suggested to regulate the gut barrier and claimed to be pre-haptoglobin 2 (pre-HP2) and circulating zonulin is often used as a proxy for gastrointestinal permeability. This study investigated the correlation between colonic paracellular permeability and levels of circulating zonulin and pre-HP2. Materials and Methods Colonic biopsies from 32 patients with IBS and 15 healthy controls (HC) were used to measure permeability in Ussing chambers and levels of zonulin (Cusabio ELISA). Zonulin was also measured in blood samples from 40 HC, 78 patients with IBS and 20 patients with celiac disease (CeD), before and after a gluten-free diet. In addition, we verified HP genotype and circulating pre-HP2 using a monoclonal pre-HP2 antibody (Bio-Rad) by ELISA. Results Increased colonic paracellular permeability correlated positively with zonulin levels in IBS biopsies, but negatively with plasma zonulin. We found no agreement between circulating zonulin and pre-HP2. Genotyping revealed non-specificity of the zonulin kit, as all pre-HP2 non-producers presented detectable levels. Patients with CeD displayed higher pre-HP2 and zonulin levels compared to HC. A gluten-free diet in patients with CeD led to lower serum zonulin and pre-HP2 concentrations. Conclusions Our study suggests that neither circulating zonulin nor pre-HP2 mirror colonic permeability. Our data corroborate previous reports showing the inability of the Cusabio zonulin kit to target zonulin and highlights that the results of studies using this kit must be re-examined with caution.

Place, publisher, year, edition, pages
Taylor & Francis, 2021. Vol. 56, no 4, p. 424-431
Keywords [en]
Colonic paracellular permeability; irritable bowel syndrome; zonulin; pre-haptoglobin 2; ELISA
National Category
Gastroenterology and Hepatology
Identifiers
URN: urn:nbn:se:liu:diva-173645DOI: 10.1080/00365521.2021.1879247ISI: 000614498300001PubMedID: 33535002Scopus ID: 2-s2.0-85100537095OAI: oai:DiVA.org:liu-173645DiVA, id: diva2:1531836
Note

Funding Agencies|Mucosa Infection and Inflammation Center-MIIC; AFA research foundation; County Council of Ostergotland; Swedish Research Council (Vetenskapsradet) (VR-Medicine and Health) [2014-02537, 2017-02475]

Available from: 2021-02-26 Created: 2021-02-26 Last updated: 2024-01-10Bibliographically approved

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Meira de Faria, FelipeBednarska, OlgaStröm, MagnusSöderholm, Johan DWalter, SusannaKeita, Åsa
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Division of Molecular Medicine and VirologyFaculty of Medicine and Health SciencesDivision of Inflammation and InfectionMag- tarmmedicinska klinikenDivision of Surgery, Orthopedics and OncologyDepartment of Surgery in Linköping
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Scandinavian Journal of Gastroenterology
Gastroenterology and Hepatology

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