The motivation to write this Handbook came from the continued and widespread interestand use of the business and technology incubation mechanisms, which have been in voguefor well over the last six decades. The interest in these ‘novel’ venture development toolshas continued to burgeon particularly over recent decades (Mian et al. 2016; Galbraithet al. 2019). Incubation mechanisms’ use as intermediaries has matured, particularlyamong the policy circles developing pro-active and dynamic knowledge and technologybasedregional entrepreneurial ecosystems; and is a well-accepted policy development (Goswami et al. 2018; Audretsch and Keilbach 2004; Audretsch et al. 2019). Their successful organizational and bridging role and professionalization of policies can be witnessedthrough better integration of the venture development activities with the surrounding environment, the acquisition of resources, thoughtful tenant recruitment, as well as a focus on societal concerns such as sustainability, green technology and equal opportunity issues (Lamine et al. 2018; Bank et al. 2017). By now, incubation mechanisms have become an integral part of the lexicon of new venture development and an essential component of any modern entrepreneurial ecosystem, supporting the growth of innovative economy based on a broad range of measures (Hausberg and Korreck 2020).