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Nitrification and Nitrifying Bacterial Communities in Coniferous Forest Soils: Effects of Liming and Clear-Cutting
Linköping University, Department of Physics, Chemistry and Biology. Linköping University, The Institute of Technology.
2003 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

This thesis deals with the effects of liming and clear-cutting on nitrification in hemi-boreal and northern temperate coniferous forest soils. The approach has been to study both the potential nitrification and the community structure of the ammonia-oxidising bacteria, which carry out the first step of autotrophic nitrification. The potential nitrification was measured over short time incubations at optimal conditions for acid-sensitive, autotrophic nitrification. This method yields the potential nitrification of the actual nitrifying community. I studied the autotrophic ammoniaoxidising community at gene level (16S rRNA gene) using molecular methods, such as polymerase chain reaction (PCR), denaturing gradient gel electrophoresis (DGGE), single-strand conformation polymorphism (SSCP), and DNA sequencing. The results illustrate that both liming and clear-cutting may increase the potential nitrification by stimulating the growth of ammonia-oxidisers. Both these forest practises seem to favour the growth of Nitrosospira cluster 4-affiliated ammonia-oxidisers, although Nitrosospira cluster 2-affiliated bacteria also was present. The stimulated growth of the ammonia-oxidisers is caused by increased ammonia availability and more favourable pH (i.e. higher and more stable pH over time). The results also show that clear-cutting causes more intense growth of the ammonia-oxidisers and thereby larger potential nitrification than liming does. When forests that have previously been limed are clearcut, nitrification responses more rapidly and the rates are larger compared to non-limed forests, since the ammonia-oxidising communities in limed soils seem better adapted to the conditions after the cutting. Liming does, however, not always increase nitrification. Although it may increase nitrogen mineralisation, it seems like the nitrogen status of the soil prior to liming is the most important factor, since liming caused the greatest response in potential nitrification in areas receiving high nitrogen deposition (>10 kg ha-1 year-1). These results suggest that although liming and clear-cutting cause similar response of the ammonia-oxidisers, the risks within creasing nitrification, such as nitrate leaching and increased emissions of the greenhouse gas nitrous oxide, are larger following clear-cutting, due to greater nitrification rates and the fact that root uptake of nitrate is interrupted.

Place, publisher, year, edition, pages
Linköping: Linköping University , 2003. , p. 74
Series
Linköping Studies in Science and Technology. Dissertations, ISSN 0345-7524 ; 809
National Category
Environmental Sciences related to Agriculture and Land-use
Identifiers
URN: urn:nbn:se:liu:diva-179553Libris ID: 8898461ISBN: 9173736198 (print)OAI: oai:DiVA.org:liu-179553DiVA, id: diva2:1597081
Public defence
2003-05-23, sal Planck, Fysikhuset, Linköpings universitet, Linköping, 10:15
Opponent
Note

All or some of the partial works included in the dissertation are not registered in DIVA and therefore not linked in this post.

Available from: 2021-09-24 Created: 2021-09-24 Last updated: 2023-02-28Bibliographically approved
List of papers
1. Increased Nitrification in Acid Coniferous Forest Soil Due to High Nitrogen Deposition and Liming
Open this publication in new window or tab >>Increased Nitrification in Acid Coniferous Forest Soil Due to High Nitrogen Deposition and Liming
2003 (English)In: Scandinavian Journal of Forest Research, ISSN 0282-7581, E-ISSN 1651-1891, Vol. 18, no 6, p. 514-524Article in journal (Refereed) Published
Abstract [en]

This study investigated whether liming stimulates the potential nitrification of acid forest soils in southern Sweden, and whether such stimulation (if present) is more pronounced in areas receiving high nitrogen (N) deposition. A short-term (30 h) soil-slurry incubation technique was used, which reduces the risk of bacterial growth, nitrate immobilization and denitrification during the incubation. The nitrate and nitrite produced were measured after biological conversion to nitrous oxide. The investigation was performed 6-7 yrs after the liming at four coniferous forest sites in the central and western parts of southern Sweden, which receive low and high deposition of N, respectively. Overall, liming had increased pH significantly down to 10 cm soil depth, but at 20 cm depth there was no difference between the limed and non-limed soil. In cases when liming had affected the total N pool and the potential nitrification, this was also limited to the uppermost 10 cm. It seems likely that the effects of liming on the potential nitrification were dependent on N availability, which is in turn influenced by N mineralization, trees' demands for N, and atmospheric N inputs. The strongest stimulatory effect of liming on the potential nitrification was seen on the west coast, indicating that these sites had the highest availability of ammonia for nitrifiers. However, liming also increased nitrification at one of the sites in south-central Sweden, which could have been mediated by increased rates of N mineralization.

Keywords
Acid forest soil, Nitrogen availability, Potential nitrification, Short-term soil-slurry incubation
National Category
Natural Sciences
Identifiers
urn:nbn:se:liu:diva-46418 (URN)10.1080/02827580310017466 (DOI)
Available from: 2009-10-11 Created: 2009-10-11 Last updated: 2021-09-24

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