liu.seSearch for publications in DiVA
Change search
ReferencesLink to record
Permanent link

Direct link
Conjugated polythiophene probes target lysosome-related acidic vacuoles in cultured primary cells
Linköping University, Department of Physics, Chemistry and Biology, Biomolecular and Organic Electronics. Linköping University, The Institute of Technology.
Linköping University, Department of Physics, Chemistry and Biology, Organic Chemistry. Linköping University, The Institute of Technology.ORCID iD: 0000-0002-5582-140X
Linköping University, Department of Clinical and Experimental Medicine, Clinical Chemistry. Linköping University, Faculty of Health Sciences.
Linköping University, Department of Clinical and Experimental Medicine, Clinical Chemistry. Linköping University, Faculty of Health Sciences.
Show others and affiliations
2007 (English)In: Molecular and Cellular Probes, ISSN 0890-8508, Vol. 21, no 5-6, 329-337 p.Article in journal (Refereed) Published
Abstract [en]

Conformation-sensitive optical probes for studying biological processes and structures are of great interest. The present work shows for the first time that conjugated polyelectrolyte (CPE) probes can be used for specific targeting of chromatin, nuclear and cytoplasmatic vesicles, and cytoskeletal components in a complex system of cultured cells. One of the probes could also be used for vital staining of live cells. When bound to different entities, the polythiophene derivative probes emitted light with different colors due to the unique spectral properties of these conformation sensitive probes. The physical pre-requisites for binding could also be exploited for characterization of the target. Unexpectedly, lysosome-related acidic vacuoles were targeted in cultured primary cells by both anionic, cationic, and zwitter-ionic polythiophene derivatives. Pre-treatment with Bafilomycin A1, a specific inhibitor of vacuolar-type H+-ATPase, caused redistribution of the staining. The targeting of lysosome-related acidic vesicles could not be demonstrated in transformed cells (melanoma, neuroblastoma, and prostate cancer cell lines), indicating a difference in the localization, structure, accessibility, or quantity of the target in cultured normal cells as compared with the malignant cell lines. The chemical nature of the conjugated polyelectrolyte complex in the cytoplasmatic vacuoles remains elusive.

Place, publisher, year, edition, pages
2007. Vol. 21, no 5-6, 329-337 p.
Keyword [en]
Conjugated polyelectrolyte; Polythiophene; Fibroblast; Lysosome; Cancer
National Category
Medical and Health Sciences
URN: urn:nbn:se:liu:diva-12727DOI: 10.1016/j.mcp.2007.04.005OAI: diva2:16929
Available from: 2007-12-07 Created: 2007-12-07 Last updated: 2014-04-08
In thesis
1. Biological Sensing and DNA Templated Electronics Using Conjugated Polymers
Open this publication in new window or tab >>Biological Sensing and DNA Templated Electronics Using Conjugated Polymers
2007 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Conjugated polymers have been found useful in a wide range of applications such as solar cells, sensor elements and printed electronics, due to their optical and electronic properties. Functionalization with charged side chains has enabled water solubility, resulting in an enhanced interaction with biomolecules. This thesis focus on the emerging research fields, where these conjugated polyelectrolytes (CPEs) are combined with biomolecules for biological sensing and DNA nanowire assembling.

CPEs have shown large potential in biomolecular detection where the optical read out is due to the geometrical alternation in the backbone and aggregation state. This thesis focused on transferring the biomolecular detection to a surface of CPEs. The characterization of the CPE layer show that a hydrogel can be formed, and how the layer can undergo geometrical changes upon external stimulus such as pH change. A selective sensor surface can be created by imprinting ssDNA or an antibody in the CPE layer. The discrimination for complementary DNA hybridization and specific antibody interaction can be monitored by surface plasmon resonance or quartz crystal microbalance. We have also taken the step out from the controlled test tube experiments to the complex environment of the cell showing the potential for staining of compartments and structures in live and fixed cell. Depending on the conditions and CPE used, cell nuclei, acidic vesicles and cytoskeleton structure can be visualized. Furthermore, the live staining shows no sign of toxic effect on cultured fibroblasts.

CPEs can also be a valuable element when assembling electronics in the true nano regime. I have used DNA as building template due to its attractive size features, with a width of around 2 nm and a length scale in the µm regime, and the inbuilt base-paring recognition elements. This thesis shows how DNA can be decorated with CPEs and stretched on surfaces into a model for aligned semiconducting nanowire geometries. Not only making the template structures is of importance, but also how to place them on the correct surface position, i.e. on electrodes. Strategies for positioning DNA nanowires using transfer printing and surface energy patterning methods have therefore been developed in the thesis. The stretched DNA decorated with CPE also offers a way to further study the molecular binding interaction between the two molecules. Single molecular spectroscopy in combination with polarization has given information of the variation of the CPE binding along a DNA chain.

Place, publisher, year, edition, pages
Institutionen för fysik, kemi och biologi, 2007
Linköping Studies in Science and Technology. Dissertations, ISSN 0345-7524 ; 1154
conjugated polymer, conjugated polyelectrolyte, DNA, sensor, nanowire
National Category
Industrial Biotechnology
urn:nbn:se:liu:diva-10180 (URN)978-91-85895-17-5 (ISBN)
Public defence
2007-12-14, Planck, Fysikhuset, Campus Valla, Linköpings Universitet, Linköping, 10:15 (English)
Available from: 2007-12-07 Created: 2007-12-07 Last updated: 2009-04-23

Open Access in DiVA

No full text

Other links

Publisher's full textLink to Ph.D. thesis

Search in DiVA

By author/editor
Björk, PerNilsson, PeterLenner, LiselotteKågedal, BertilPersson, BirgittaInganäs, OlleJonasson, Jon
By organisation
Biomolecular and Organic ElectronicsThe Institute of TechnologyOrganic ChemistryClinical ChemistryFaculty of Health SciencesDepartment of Biomedicine and SurgeryMolecular and Immunological PathologyDepartment of Clinical Pathology and Clinical Genetics
Medical and Health Sciences

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 152 hits
ReferencesLink to record
Permanent link

Direct link