Phosphorylation of IRS1 at serine 307 and serine 312 in response to insulin in human adipocytes
2006 (English)In: Biochemical and biophysical research communications, ISSN 0006-291X, Vol. 342, no 4, 1183-1187 p.Article in journal (Refereed) Published
Feedback control in insulin signaling involves serine phosphorylation of insulin receptor substrate-1 (IRS1). By analyzing the insulin-induced phosphorylation of IRS1 at serine 307, serine 312, and tyrosine in the same primary human adipocytes, we now report that negative feedback phosphorylation of serine 312 (corresponding to murine serine 307) required relatively high concentrations of insulin (EC50 = 3 nM) for a long time (t1/2 ca. 30 min) and reduced the steady-state tyrosine phosphorylation, without affecting the cellular concentration, of IRS1. In contrast, positive feedback phosphorylation of serine 307 was a rapid (t1/2 ca. 2 min) event at physiological concentrations of insulin (EC50 = 0.2 nM).
Place, publisher, year, edition, pages
2006. Vol. 342, no 4, 1183-1187 p.
Insulin; Insulin resistance; Insulin receptor substrate-1; Serine phosphorylation; Tyrosine phosphorylation; Positive feedback; Negative feedback
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:liu:diva-12792DOI: 10.1016/j.bbrc.2006.02.075OAI: oai:DiVA.org:liu-12792DiVA: diva2:17064