Importance of polymorphisms at NF-κB1 and NF-κBIα genes in melanoma risk, clinicopathological features and tumor progression in Swedish melanoma patients
2007 (English)In: Journal of Cancer Research and Clinical Oncology, ISSN 0171-5216, Vol. 133, no 11, 859-866 p.Article in journal (Refereed) Published
In this study, functional polymorphisms of NF-κB1 and NF-κBIα genes were examined in 185 melanoma patients and 438 tumor-free individuals. Associations of the polymorphisms with melanoma risk, age and pigment phenotypes of the patients and clinico-pathological tumor characteristics were analyzed. DNAs were isolated from mononuclear cells of venous blood. Polymorphisms of the genes were genotyped by a PCR-RFLP technique, and transcription level of NF-κBIα was examined by a quantitative real-time reverse transcription PCR. Results showed that both ATTG insertion polymorphism of NF-κB1 and A to G polymorphism of NF-κBIα genes were correlated with melanoma risk, especially, in a combination of ATTG2/ATTGT2 and GG. NF-κB1 ATTG2/ATTG2 and NF-κBIα GG genotypes were associated with male gender and age > 65 years (at diagnosis). Patients with ATTG1/ATTG1 genotype had thinner tumors and lower Clark levels at diagnosis. Frequency of ATTG1/ATTG1 genotype was higher in patients with melanomas on intermittently sun-exposed pattern of the body and NF-κBIα GG was more frequent in the patients with melanomas at rarely exposed sites. There were no differences in the gene transcription level between patients with different NF-κBIα genotypes. These data suggest that NF-κB1 and NF-κBIα genes might be susceptible genes for melanoma risk and functional polymorphisms of these genes might be biological predictors for melanoma progression.
Place, publisher, year, edition, pages
Institutionen för biomedicin och kirurgi , 2007. Vol. 133, no 11, 859-866 p.
polymorphisms, NF-κB1, NF-κBIα, melanoma risk, clinicopathological features, phenotypes
IdentifiersURN: urn:nbn:se:liu:diva-10580DOI: 10.1007/s00432-007-0228-7OAI: oai:DiVA.org:liu-10580DiVA: diva2:17315
The original publication is available at www.springerlink.com: Huajie Bu, Inger Rosdahl, XiaoFeng Sun, Katarina Holmdahl-Källenand and Hong Zhang, Importance of polymorphisms at NF-κB1 and NF-κBIα genes in melanoma risk, clinicopathological features and tumor progression in Swedish melanoma patients, 2007, Journal of Cancer Research and Clinical Oncology, (133), 11, 859-866. http://dx.doi.org/10.1007/s00432-007-0228-7 . Copyright: Springer-Verlag, www.springerlink.com2008-05-122008-05-122009-08-17