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Oligothiophene Assemblies Defined by DNA Interaction: From Single Chains to Disordered Clusters
Linköping University, Department of Physics, Chemistry and Biology, Biomolecular and Organic Electronics . Linköping University, The Institute of Technology.
Lund University.
Lund University.
Linköping University, Department of Physics, Chemistry and Biology, Biomolecular and Organic Electronics . Linköping University, The Institute of Technology.
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2009 (English)In: SMALL, ISSN 1613-6810 , Vol. 5, no 1, 96-103 p.Article in journal (Refereed) Published
Abstract [en]

The organization of conjugated polyelectrolytes (CPEs) interacting with biomolecules sets conditions for the biodetection of biological processes and identity, through the use of optical emission from the CPE. Herein, a well-defined CPE and its binding to DNA is studied. By using dynamic light scattering and circular dichroism spectroscopy, it is shown that the CPE forms a multimolecule ensemble in aqueous solution that is more than doubled it? size when interacting with a small DNA chain, while single chains are evident in ethanol. The related changes in the fluorescence spectra upon polymer aggregation are assigned to oscillator strength redistribution between vibronic transitions in weakly coupled H-aggregates. An enhanced single-molecule spectroscopy technique that allows full control of excitation and emission light polarization is applied to combed and decorated;,DNA chains. It is found that the organization of combed CPE-lambda DNA complexes (when dry on the surface) allows considerable variation of CPE distances and direction relative to the DNA chain. By analysis of the polarization data. energy transfer between the polymer chains in individual complexes is confirmed and their sizes estimated.

Place, publisher, year, edition, pages
2009. Vol. 5, no 1, 96-103 p.
Keyword [en]
aggregation, conjugated polymers, DNA, fluorescence, single-molecule spectroscopy
National Category
Natural Sciences
Identifiers
URN: urn:nbn:se:liu:diva-16829DOI: 10.1002/smll.200800855OAI: oai:DiVA.org:liu-16829DiVA: diva2:174356
Available from: 2009-02-20 Created: 2009-02-20 Last updated: 2010-04-20
In thesis
1. Conjugated Polyelectrolytes in Interactions with Biomolecules for Supramolecular assembly and Sensing
Open this publication in new window or tab >>Conjugated Polyelectrolytes in Interactions with Biomolecules for Supramolecular assembly and Sensing
2010 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Conjugated polyelectrolytes (CP) show interesting electrical and optical properties for organic electronics as well as for life science applications. Their possibilities of supramolecular assembly with nanowire like misfolded proteins, amyloids, as well as synthetic polypeptides or DNA forming conducting or luminescent nano composites is highly interesting as being a truly bottom up approach for fabrication of OLEDs, photovoltaic’s as well as logic devices. The conformation and aggregation dependent luminescence properties from the special class of CPs, Luminescent conjugated polyelectrolytes (LCP), have been utilised and developed as sensors to follow and study biomolecular interactions, DNA hybridisation, protein-protein interactions and staining of living cell cultures and tissue slides. In this thesis we are bringing the evolution a few steps further by applying new types of experimental techniques, such as light scattering and fluorescence correlation spectroscopy, combined with standard techniques as soft lithography and different spectroscopy techniques, to gain better knowledge of the optical behaviour of LCPs and their interactions with biomolecules. We explore the optical properties and vibronic transitions of LCPs; their ability of resonance energy transfer with LCPs indicating super lightning behaviour; the opposite fluorescence shift when interacting with α-helical rich polypeptides compared to earlier reports of interactions upon staining of β-rich amyloids; and the possibility of LCPs to influence protein aggregation as well as the possibility of fabricating biochips based on LCPs and soft lithography. Here we also show fundamental limitations to patterning using macromolecular fluids, of general relevance to soft lithography and nanoimprint lithography with low viscosity polymers.

Place, publisher, year, edition, pages
Linköping: Linköping University Electronic Press, 2010. 60 p.
Series
Linköping Studies in Science and Technology. Dissertations, ISSN 0345-7524 ; 1308
National Category
Natural Sciences
Identifiers
urn:nbn:se:liu:diva-54902 (URN)978-91-7393-408-4 (ISBN)
Public defence
2010-05-14, Planck, Fysikhuset, Campus Valla, Linköpings universitet, Linköping, 14:00 (English)
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Available from: 2010-04-20 Created: 2010-04-20 Last updated: 2014-04-08Bibliographically approved

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Björk, PerAndersson, JensInganäs, Olle

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