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Synthesis and Characterization of Tb3+-Doped Gd2O3 Nanocrystals: A Bifunctional Material with Combined Fluorescent Labeling and MRI Contrast Agent Properties
Linköping University, Department of Physics, Chemistry and Biology, Molecular Surface Physics and Nano Science. Linköping University, Faculty of Science & Engineering.
Linköping University, Department of Physics, Chemistry and Biology. Linköping University, The Institute of Technology.
Linköping University, Center for Medical Image Science and Visualization (CMIV). Linköping University, Department of Medical and Health Sciences, Radiology. Linköping University, Faculty of Health Sciences. Östergötlands Läns Landsting, Centre for Medical Imaging, Department of Radiology in Linköping.
Linköping University, Department of Physics, Chemistry and Biology, Applied Physics. Linköping University, Faculty of Science & Engineering.
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2009 (English)In: The Journal of Physical Chemistry C, ISSN 1932-7447, E-ISSN 1932-7455, Vol. 113, no 17, 6913-6920 p.Article in journal (Refereed) Published
Abstract [en]

Ultrasmall gadolinium oxide nanoparticles doped with terbium ions were synthesized by the polyol route and characterized as a potentially bifunctional material with both fluorescent and magnetic contrast agent properties. The structural, optical, and magnetic properties of the organic-acid-capped and PEGylated Gd2O3:Tb3+ nanocrystals were studied by HR-TEM, XPS, EDX, IR, PL, and SQUID. The luminescent/fluorescent property of the particles is attributable to the Tb3+ ion located on the crystal lattice of the Gd2O3 host. The paramagnetic behavior of the particles is discussed. Pilot studies investigating the capability of the nanoparticles for fluorescent labeling of living cells and as a MRI contrast agent were also performed. Cells of two cell lines (THP-1 cells and fibroblasts) were incubated with the particles, and intracellular particle distribution was visualized by confocal microscopy. The MRI relaxivity of the PEGylated nanoparticles in water at low Gd concentration was assessed showing a higher T-1 relaxation rate compared to conventional Gd-DTPA chelates and comparable to that of undoped Gd2O3 nanoparticles.

Place, publisher, year, edition, pages
2009. Vol. 113, no 17, 6913-6920 p.
National Category
Natural Sciences
Identifiers
URN: urn:nbn:se:liu:diva-12944DOI: 10.1021/jp808708mISI: 000265529700009OAI: oai:DiVA.org:liu-12944DiVA: diva2:17498
Note

On the day of the defence date the status of this article was Submitted

Available from: 2008-02-21 Created: 2008-02-21 Last updated: 2017-12-13Bibliographically approved
In thesis
1. MRI Contrast Enhancement using Gd2O3 Nanoparticles
Open this publication in new window or tab >>MRI Contrast Enhancement using Gd2O3 Nanoparticles
2008 (English)Licentiate thesis, comprehensive summary (Other academic)
Abstract [en]

There is an increasing interest for nanomaterials in biomedical applications and in this work, nanoparticles of gadolinium oxide (Gd2O3) have been investigated as a novel contrast agent for Magnetic Resonance Imaging (MRI). Relaxation properties have been studied in aqueous solutions as well as in cell culture medium and the nanoparticles have been explored as cell labeling agents. The fluorescent properties of the particles were used to visualize the internalization in cells and doped particles were also investigated as a multimodal agent that could work as a fluorescent marker for microscopy and as a contrast enhancer for MRI.

Results show that in aqueous solutions, there is a twofold increase in relaxivity for Gd2O3 compared to commercial agent Gd-DTPA. In cell culture medium as well as in cells, there is a clear T1 effect and a distinct increase in signal intensity in T1-mapped images. Fluorescent studies show that the Gd2O3 nanoparticles doped with 5% terbium have interesting fluorescent properties and that these particles could work as a multimodal contrast agent.

This study shows that Gd2O3 nanoparticles possess excellent relaxation properties that are retained in more biological environments. Gd2O3 particles are suitable as a T1 contrast agent, but seem also be adequate for T2 enhancement in for instance cell labeling experiments.

Place, publisher, year, edition, pages
Universitetsbibliotek, 2008. 116 p.
Series
Linköping Studies in Health Sciences. Thesis, ISSN 1100-6013 ; 85
Keyword
nanoparticles, gadolinium oxide, magnetic resonance imaging, contrast agent
National Category
Clinical Science
Identifiers
urn:nbn:se:liu:diva-11041 (URN)978-91-7393-966-9 (ISBN)
Presentation
2008-03-07, Conrad, Huvudblocket, plan 11, Campus US, Linköpings universitet, Linköping, 13:00 (English)
Supervisors
Available from: 2008-02-21 Created: 2008-02-21 Last updated: 2013-09-04
2. MRI Contrast Enhancement and Cell Labeling using Gd2O3 Nanoparticles
Open this publication in new window or tab >>MRI Contrast Enhancement and Cell Labeling using Gd2O3 Nanoparticles
2011 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

There is an increasing interest for nanomaterials in bio-medical applications and in this work, nanoparticles of gadolinium oxide (Gd2O3 ) have been investigated as a novel contrast agent for magnetic resonance imaging (MRI). Relaxation properties have been studied in aqueous solutions as well as in cell culture medium and the nanoparticles have been explored as cell labeling agents. The fluorescent properties of the particles were used to visualize the internalization in cells and doped particles were investigated as a multimodal agent that could work as a fluorescent marker for microscopy and as a contrast enhancer for MRI. Fluorescent studies show that the Gd2O3 nanoparticles doped with 5% terbium have interesting fluorescent properties and that these particles could work as such multimodal contrast agent. Relaxivity measurements show that in aqueous solutions, there is a twofold increase in relaxivity for Gd2O3 compared to commercial agent Gd-DTPA. In cell culture medium as well as in cells, there is a clear T1 effect and an increase in signal intensity in T1-mapped images. The cellular uptake of Gd2O3 nanoparticles were increased with the use of transfection agent protamine sulfate. This work shows that Gd2O3 nanoparticles possess good relaxation properties that are retained in different biological environments. Gd2O3 particles are suitable as a T1 contrast agent, but seem also be adequate for T2 enhancement in forinstance cell labeling experiments.

Place, publisher, year, edition, pages
Linköping: Linköping University Electronic Press, 2011. 73 p.
Series
Linköping University Medical Dissertations, ISSN 0345-0082 ; 1230
National Category
Medical and Health Sciences
Identifiers
urn:nbn:se:liu:diva-68802 (URN)978-91-7393-215-8 (ISBN)
Public defence
2011-06-08, Wrannesalen, Universitetssjukhuset, CMIV, Campus US, Linköpings universitet, Linköping, 09:00 (Swedish)
Opponent
Supervisors
Available from: 2011-06-07 Created: 2011-06-07 Last updated: 2013-09-05Bibliographically approved

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Petoral, Rodrigo MKlasson, AnnaSuska, AnkeFortin, Marc-AndréAbrikossova, NataliaKäll, Per-OlovEngström, MariaUvdal, Kajsa

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Petoral, Rodrigo MKlasson, AnnaSuska, AnkeFortin, Marc-AndréAbrikossova, NataliaKäll, Per-OlovEngström, MariaUvdal, Kajsa
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Molecular Surface Physics and Nano ScienceFaculty of Science & EngineeringDepartment of Physics, Chemistry and BiologyThe Institute of TechnologyCenter for Medical Image Science and Visualization (CMIV)RadiologyFaculty of Health SciencesDepartment of Radiology in LinköpingApplied PhysicsSensor Science and Molecular PhysicsPhysical Chemistry
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