Lysosome-targeted stress reveals increased stability of lipofuscin-containing lysosomes
2008 (English)In: Age (Omaha), ISSN 0161-9152, Vol. 30, no 1, 31-42 p.Article in journal (Refereed) Published
Cellular ageing is associated with accumulation of undegradable intralysosomal material, called lipofuscin. In order to accelerate the lipofuscin-accumulation, confluent, growth arrested human fibroblasts were cultured under hyperoxic conditions. To provide a better insight into the effects of lipofuscin on cellular functions, we compared lysosomal stability in control and lipofuscin-loaded human fibroblasts under conditions of lysosome-targeted stress induced by exposure to either the lysosomotropic detergent MSDH or the redox-cycling quinone naphthazarin. We show that lysosomal damage, assessed by acridine-orange relocation, translocation of cathepsin D to the cytosol, and alkalinization of lysosomes is more pronounced in control than in lipofuscin-loaded fibroblasts. Finding that lysosomal integrity was less affected or even preserved in case of lipofuscin-loaded cells enables us to suggest that lipofuscin exerts lysosome-stabilizing properties.
Place, publisher, year, edition, pages
Institutionen för klinisk och experimentell medicin , 2008. Vol. 30, no 1, 31-42 p.
alkalinization, autophagolysosomes, bafilomycin A1, cathepsin D, MSDH, naphthazarin quinone
IdentifiersURN: urn:nbn:se:liu:diva-11051DOI: 10.1007/s11357-007-9045-9OAI: oai:DiVA.org:liu-11051DiVA: diva2:17508
Note: Because of an embargo period with Springer journals, the full text will become available at LiU E-Press, January 2009. Original publication: Yuri Stroikin, Hanna Mild, Uno Johansson, Karin Roberg and Karin Öllinger, Lysosome-targeted stress reveals increased stability of lipofuscin-containing lysosomes, 2008, AGE http://dx.doi.org/10.1007/S11357-007-9045-9. Copyright: The original publication is available at http://www.springerlink.com2009-01-122008-04-152009-08-21