Elongation of Actin Upon Binding to Prefoldin
(English)Manuscript (Other (popular scientific, debate etc.))
This pilot study on the interaction between actin andprefoldin indicates that actin binds to prefoldin in astretched conformation, probably similar to the bindingconformation at the initial interaction with TRiC/ADP as shown in a previous study (8). Further refinement of theprefoldin purification is a future aim, as well as additionalhomo-FRET measurements on several actin variantsspread throughout the structure to investigate the localvolume expansions/compressions of the prefoldin-boundactin molecule and compare the results to actin bound tothe chaperonins TRiC/ADP, TRiC/AMP-PNP and GroEL(8, 18). A fairly detailed comparison between thedistances throughout the actin molecule when bound toprefoldin and TRiC/ADP could reveal if the stretching inprefoldin is the same as in TRiC/ADP, or if there isadditional effects on the actin molecule as it is beingtransferred from prefoldin to the TRiC cavity.
IdentifiersURN: urn:nbn:se:liu:diva-13127OAI: oai:DiVA.org:liu-13127DiVA: diva2:17876