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Biosensor discrimination of meat juice from various animals using a lectin panel and ellipsometry
Linköping University, Department of Physics, Chemistry and Biology, Applied Physics . Linköping University, The Institute of Technology. (Applied Physics)
Linköping University, Department of Physics, Chemistry and Biology, Applied Physics . Linköping University, The Institute of Technology.
Pure and Applied Biochemistry, Lund University, SE-22 100 Lund, Sweden .
Linköping University, Department of Physics, Chemistry and Biology, Applied Physics . Linköping University, The Institute of Technology.
2005 (English)In: Analytica Chimica Acta, ISSN 0003-2670, E-ISSN 1873-4324, Vol. 547, no 2, 229-236 p.Article in journal (Refereed) Published
Abstract [en]

In this work, simple microcontact printed gold-wafers were used to make a lectin panel for investigation and discrimination of different meat juices from fresh meat of cattle, chicken, pig, cod, turkey and lamb. Seven different lectins were thus attached to gold surfaces using the streptavidin–biotin method. Lectins recognize and bind specifically to carbohydrate structures present on different proteins. The biorecognition was evaluated with null ellipsometry and the data obtained was related to an internal standard of lactoferrin. The data was evaluated with multivariate data analysis techniques to identify possible discrimination or grouping of data. Scanning ellipsometry was used for visualization of the binding pattern of the lectins and the meat juice proteins. The two-dimensional images obtained could be used to visualize the protein distribution, furthermore, to exclude anomalies. The results showed that the different meat juices from the six different species: cattle, chicken, pig, cod, turkey and lamb could be discriminated from each other. The results showed to be more repetitive for the mammalian meat juices. Using a simple model based on an artificial neuronal net, it was also possible to classify meat juices from the mammals investigated.

Place, publisher, year, edition, pages
2005. Vol. 547, no 2, 229-236 p.
Keyword [en]
Lectin panel; Ellipsometry; Meat juice; Multivariate data analysis
National Category
Chemical Sciences
Identifiers
URN: urn:nbn:se:liu:diva-12467DOI: 10.1016/j.aca.2005.05.054OAI: oai:DiVA.org:liu-12467DiVA: diva2:179
Available from: 2008-09-06 Created: 2008-09-06 Last updated: 2017-12-13Bibliographically approved
In thesis
1. Interaction Studies in Complex Fluids with Optical Biosensors
Open this publication in new window or tab >>Interaction Studies in Complex Fluids with Optical Biosensors
2008 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

In this thesis interactions in complex fluids, such as serum and meat juice, were analysed with optical biosensor techniques.

Panels of lectins immobilised on gold surfaces were used for investigation of differences in protein glycosylation pattern in sera and meat juices between various species. The present panel was also used for investigation of global glycosylation changes of serum proteins in type 1 diabetes patients. Biorecognition was evaluated with null ellipsometry and scanning ellipsometry combined with multivariate data analysis techniques (MVDA). Principal component analysis (PCA) showed that the lectin panel enabled discrimination between sera from the different species as well as for the different meat juices. The results also indicate that there is a measurable global alteration in glycosylation pattern of serum proteins in type 1 diabetic patients compared to healthy subjects. Using an artificial neuronal net (ANN), it was also possible to correctly categorise unknown serum samples into their respective class or group. The analytical potential of combining information from lectin panels with multivariate data analysis was thereby demonstrated.

Also, a sensitive and specific method based on surface plasmon resonance (SPR) for detection of insulin autoantibodies (IAA) in serum samples from individuals at high risk of developing type 1 diabetes (T1D) has been developed. When measuring trace molecules, such as autoantibodies, in undiluted sera with label-free techniques like SPR, non-specific adsorption of matrix proteins to the sensor surface is often a problem, since it causes a signal that masks the analyte response. The developed method is an indirect competitive immunoassay designed to overcome these problems. Today, IAA is mainly measured in radio immunoassays (RIAs), which are time consuming and require radioactively labelled antigen. With our SPR-based immunoassay the overall assay time is reduced by a factor of >100 (from 4 days to 50 min), while sensitivity is maintained at a level comparable to that offered by RIA. Finally, the assay was used in a screening study of newly diagnosed type 1 diabetes patients and non-diabetic subjects.

Place, publisher, year, edition, pages
Linköping: Linköping University Electronic Press, 2008. 70 p.
Series
Linköping Studies in Science and Technology. Dissertations, ISSN 0345-7524 ; 1195
Keyword
Type 1 diabetes, Insulin, Lectin panel, Ellipsometry, Meat juice, Serum proteins
National Category
Chemical Sciences
Identifiers
urn:nbn:se:liu:diva-14694 (URN)978-91-7394-852-5 (ISBN)
Public defence
2008-09-19, Planck, Fysikhuset, Campus Norrköping, Linköpings universitet, Linköping, 10:15 (English)
Opponent
Supervisors
Available from: 2008-09-26 Created: 2008-09-19 Last updated: 2009-04-24Bibliographically approved

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Carlsson, JennyWinquist, FredrikLundström, Ingemar

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