liu.seSearch for publications in DiVA
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • oxford
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Elevation of systemic matrix metalloproteinase-2 and -7 and tissue inhibitor of metalloproteinases-2 in patients with a history of Achilles tendon rupture
Linköping University, Department of Clinical and Experimental Medicine, Orthopaedics and Sports Medicine. Linköping University, Faculty of Health Sciences.
Linköping University, Department of Clinical and Experimental Medicine. Linköping University, Faculty of Health Sciences.
Linköping University, Department of Clinical and Experimental Medicine, Orthopaedics and Sports Medicine. Linköping University, Faculty of Health Sciences.
Linköping University, Department of Clinical and Experimental Medicine, Orthopaedics and Sports Medicine. Linköping University, Faculty of Health Sciences. Östergötlands Läns Landsting, Centre for Surgery, Orthopaedics and Cancer Treatment, Department of Orthopaedics in Linköping.
2010 (English)In: British Journal of Sports Medicine, ISSN 0306-3674, E-ISSN 1473-0480, Vol. 38, 308-317 p.Article in journal (Refereed) Published
Abstract [en]

Objectives: To compare serum levels of matrix metalloproteinases (MMPs) and tissue inhibitors of metalloproteinases (TIMPs) between patients with a history of Achilles tendon rupture and blood donor controls, and to relate MMPs and TIMPs to mechanical properties of the tendons during healing.

Methods: More than three years after injury, we measured serum levels of MMP-1, -2, -3, -7, -8, -9 and -13 and TIMP-1 and -2 in eight patients who had suffered Achilles tendon rupture. Twelve blood donors served as controls. During the early phase of healing, the tendon modulus of elasticity was calculated from radiostereometric data and tendon cross-sectional area.

Results: Patients with a history of Achilles tendon rupture had increased levels of MMP-2 (median difference (m.d.) 10 %; p = 0.01), MMP-7 (m.d. 15 %; p = 0.02) and TIMP-2 (m.d. 36%; p = 0.02), as compared to controls. Levels of MMP-7, measured three years after injury, correlated inversely to tendon modulus of elasticity (rs = -0.83; p = 0.02), and positively to tendon elongation (rs = 0.74; p = 0.05) during the early phase of healing. There was a trend towards positive correlation between MMP-7 and cross-sectional area during the early phase of healing (rs = 0.67; p = 0.08).

Conclusions: Patients with a history of Achilles tendon rupture appear to have elevated levels of MMP-2, MMP-7 and TIMP-2 in serum. These pilot data support the view that the MMP-TIMP system is involved in tendinopathy and suggest that disturbances in proteolytic control might be generalised.

Place, publisher, year, edition, pages
2010. Vol. 38, 308-317 p.
National Category
Medical and Health Sciences
Identifiers
URN: urn:nbn:se:liu:diva-13151DOI: 10.1136/bjsm.2008.049411OAI: oai:DiVA.org:liu-13151DiVA: diva2:17918
Available from: 2008-05-07 Created: 2008-05-07 Last updated: 2017-12-13
In thesis
1. Towards surgical use of matrix metalloproteinase biology
Open this publication in new window or tab >>Towards surgical use of matrix metalloproteinase biology
2008 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Matrix metalloproteinases (MMPs), such as collagenases, are a family of enzymes capable of degrading most constituents of the extracellular matrix. MMPs are thought to be involved in the aetiopathogenesis of tendon rupture. Additionally, failure of healing has in some instances been associated with elevated levels of MMPs. We have studied (a) the effects of the MMP-inhibitor doxycycline on healing of tendons and intestines in experimental models and (b) systemic levels of MMPs and their endogenous inhibitors (TIMPs) in patients with tendon rupture.

In the first study, systemic doxycycline treatment lead to weakened rat Achilles tendons during healing after injury.

Subsequently, systemic doxycycline was shown to improve biomechanical properties of tendon suture fixation in the rat Achilles tendon. Sutures were also coated with doxycycline, leading to similar improvement in mechanical strength of the suture construct during healing.

In the third study, doxycycline-coated sutures improved the strength of healing intestinal anastomoses in an experimental model.

Finally, we showed that patients with a history of Achilles tendon rupture had elevated levels of MMP-2, MMP-7 and TIMP-2 in serum. In addition, MMP-7 correlated inversely to mechanical strength of the tendon during healing.

In conclusion, MMP-inhibitors can be administered systemically and locally to manipulate healing of tendons and intestines. Generalised alterations in the MMP-TIMP system may be involved in the pathogenesis of Achilles tendon rupture and associated with differences in outcome of healing.

Place, publisher, year, edition, pages
Linköping University Electronic Press, 2008. 61 p.
Series
Linköping University Medical Dissertations, ISSN 0345-0082 ; 1057
Keyword
Achilles tendon, colon, tetracyclines, tissue inhibitor of metalloproteinases (TIMP), wound healing., colorectal surgery, extracellular matrix, humans, matrix metalloproteinases (MMPs), rats, surgical anastomosis, sutures, tendon injuries
National Category
Surgery
Identifiers
urn:nbn:se:liu:diva-11489 (URN)978-91-7393-931-7 (ISBN)
Public defence
2008-05-09, Linden, Hälsonuniversitetet, Linköping, 13:30 (English)
Opponent
Supervisors
Available from: 2008-05-07 Created: 2008-05-07 Last updated: 2015-11-19

Open Access in DiVA

No full text

Other links

Publisher's full text

Authority records BETA

Pasternak, BjörnSchepull, ThorstenEliasson, PernillaAspenberg, Per

Search in DiVA

By author/editor
Pasternak, BjörnSchepull, ThorstenEliasson, PernillaAspenberg, Per
By organisation
Orthopaedics and Sports MedicineFaculty of Health SciencesDepartment of Clinical and Experimental MedicineDepartment of Orthopaedics in Linköping
In the same journal
British Journal of Sports Medicine
Medical and Health Sciences

Search outside of DiVA

GoogleGoogle Scholar

doi
urn-nbn

Altmetric score

doi
urn-nbn
Total: 229 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • oxford
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf