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Detection of global glycosylation changes of serum proteins in type 1 diabetes using a lectin panel and multivariate data analysis
Linköping University, Department of Physics, Chemistry and Biology, Applied Physics . Linköping University, The Institute of Technology.
Linköping University, Department of Clinical and Experimental Medicine, Pediatrics . Linköping University, Faculty of Health Sciences.
Linköping University, Department of Clinical and Experimental Medicine, Pediatrics . Linköping University, Faculty of Health Sciences. Östergötlands Läns Landsting, Centre of Paediatrics and Gynecology and Obstetrics, Department of Paediatrics in Linköping.
Linköping University, Department of Physics, Chemistry and Biology, Applied Physics . Linköping University, The Institute of Technology.
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2008 (English)In: Talanta: The International Journal of Pure and Applied Analytical Chemistry, ISSN 0039-9140, Vol. 76, no 2, 333-337 p.Article in journal (Refereed) Published
Abstract [en]

Global glycosylation changes of serum proteins in type 1 diabetic patients have in this paper been investigated based on the interaction of the saccharide moiety of serum proteins with different lectins. Lectins are proteins, which bind carbohydrates specifically and reversibly. Panels with lectins of various carbohydrate specificities were immobilized on gold surfaces. Sera from healthy individuals, newly diagnosed type 1 diabetes patients and type 1 diabetes patients having had the disease for 4–6 years, respectively, were applied to the lectin panel. The biorecognition was evaluated with null ellipsometry. Data obtained were related to an internal standard of lactoferrin. Multivariate data analysis (MVDA) techniques were used to analyze data.

Principal component analysis showed that the lectin panel enabled discrimination between sera from the three different above-mentioned groups. Using an artificial neuronal net (ANN), it was possible to correctly categorize unknown serum samples into one of the three groups.

 

Place, publisher, year, edition, pages
2008. Vol. 76, no 2, 333-337 p.
Keyword [en]
Lectin panel, Glycosylation changes, Type 1 diabetes, Ellipsometry, MVDA
National Category
Chemical Sciences
Identifiers
URN: urn:nbn:se:liu:diva-12468DOI: 10.1016/j.talanta.2008.02.046OAI: oai:DiVA.org:liu-12468DiVA: diva2:180
Available from: 2008-09-06 Created: 2008-09-06 Last updated: 2013-06-12Bibliographically approved
In thesis
1. Interaction Studies in Complex Fluids with Optical Biosensors
Open this publication in new window or tab >>Interaction Studies in Complex Fluids with Optical Biosensors
2008 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

In this thesis interactions in complex fluids, such as serum and meat juice, were analysed with optical biosensor techniques.

Panels of lectins immobilised on gold surfaces were used for investigation of differences in protein glycosylation pattern in sera and meat juices between various species. The present panel was also used for investigation of global glycosylation changes of serum proteins in type 1 diabetes patients. Biorecognition was evaluated with null ellipsometry and scanning ellipsometry combined with multivariate data analysis techniques (MVDA). Principal component analysis (PCA) showed that the lectin panel enabled discrimination between sera from the different species as well as for the different meat juices. The results also indicate that there is a measurable global alteration in glycosylation pattern of serum proteins in type 1 diabetic patients compared to healthy subjects. Using an artificial neuronal net (ANN), it was also possible to correctly categorise unknown serum samples into their respective class or group. The analytical potential of combining information from lectin panels with multivariate data analysis was thereby demonstrated.

Also, a sensitive and specific method based on surface plasmon resonance (SPR) for detection of insulin autoantibodies (IAA) in serum samples from individuals at high risk of developing type 1 diabetes (T1D) has been developed. When measuring trace molecules, such as autoantibodies, in undiluted sera with label-free techniques like SPR, non-specific adsorption of matrix proteins to the sensor surface is often a problem, since it causes a signal that masks the analyte response. The developed method is an indirect competitive immunoassay designed to overcome these problems. Today, IAA is mainly measured in radio immunoassays (RIAs), which are time consuming and require radioactively labelled antigen. With our SPR-based immunoassay the overall assay time is reduced by a factor of >100 (from 4 days to 50 min), while sensitivity is maintained at a level comparable to that offered by RIA. Finally, the assay was used in a screening study of newly diagnosed type 1 diabetes patients and non-diabetic subjects.

Place, publisher, year, edition, pages
Linköping: Linköping University Electronic Press, 2008. 70 p.
Series
Linköping Studies in Science and Technology. Dissertations, ISSN 0345-7524 ; 1195
Keyword
Type 1 diabetes, Insulin, Lectin panel, Ellipsometry, Meat juice, Serum proteins
National Category
Chemical Sciences
Identifiers
urn:nbn:se:liu:diva-14694 (URN)978-91-7394-852-5 (ISBN)
Public defence
2008-09-19, Planck, Fysikhuset, Campus Norrköping, Linköpings universitet, Linköping, 10:15 (English)
Opponent
Supervisors
Available from: 2008-09-26 Created: 2008-09-19 Last updated: 2009-04-24Bibliographically approved

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Carlsson, JennyGullstrand, CamillaLudvigsson, JohnnyLundström, IngemarWinquist, Fredrik

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Carlsson, JennyGullstrand, CamillaLudvigsson, JohnnyLundström, IngemarWinquist, Fredrik
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Applied Physics The Institute of TechnologyPediatrics Faculty of Health SciencesDepartment of Paediatrics in Linköping
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Talanta: The International Journal of Pure and Applied Analytical Chemistry
Chemical Sciences

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