liu.seSearch for publications in DiVA
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • oxford
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Determination of antibody affinity and kinetic binding constants in Gyrolab Bioaffy microfluidic CD
Linköping University, The Department of Physics, Chemistry and Biology.
2008 (English)Independent thesis Basic level (professional degree), 20 points / 30 hpStudent thesis
Abstract [en]

Studies of binding reactions are of highest importance in a vast number of areas of biomedicine and biotechnology. A demand for fast and accurate small-volume measurements grows stronger, partly due to the development of therapeutic antibodies. In this report, a novel method for studies of binding reactions of antibodies is described. The use of a microfluidic platform shows promising results in determination of affinity binding constants.

Affinities between 1E-09 and 1E-11 M have been determined for four TSH antibodies. Reproducibility tests give a CV below 10%, using different Gyrolab instruments and microfluidic CD:s. The method carries the advantages of using solution-based measurements of unmodified molecules. Also an initial proof-of-concept for measurement of binding reaction rate constants shows further usage of the method. The kinetic association rate constant has been determined to 2E+06 M-1s-1 for one antibody. The possibility of using this method for screening of antibody libraries is also discussed.

Place, publisher, year, edition, pages
Institutionen för fysik, kemi och biologi , 2008. , 59 p.
Keyword [en]
Affinity constants, Equilibrium dissociation constants, Kinetic rate constants, Immunoassay, Monoclonal antibodies, Microfluidics, Gyrolab Bioaffy, Microlaboratory, Compact disc
National Category
Industrial Biotechnology
Identifiers
URN: urn:nbn:se:liu:diva-11616ISRN: LITH-IFM-A-EX-08/1931-SEOAI: oai:DiVA.org:liu-11616DiVA: diva2:18035
Presentation
2008-04-01, Planck, Fysikhuset, IFM / Linköpings Universitet, Linköping, 15:15
Uppsok
teknik
Supervisors
Examiners
Available from: 2008-04-29 Created: 2008-04-29

Open Access in DiVA

fulltext(1567 kB)7410 downloads
File information
File name FULLTEXT01.pdfFile size 1567 kBChecksum MD5
54342eb612e7e9073099ced9a4bcf5ef63473bb4e99bcc3737f587da95b006832281e0fa
Type fulltextMimetype application/pdf

By organisation
The Department of Physics, Chemistry and Biology
Industrial Biotechnology

Search outside of DiVA

GoogleGoogle Scholar
Total: 7410 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

urn-nbn

Altmetric score

urn-nbn
Total: 855 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • oxford
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf