An indirect competitive immunoassay for insulin autoantibodies based on surface plasmon resonance
2008 (English)In: Biosensors and Bioelectronics, ISSN 0956-5663, Vol. 24, no 4, 876-881 p.Article in journal (Refereed) Published
We have developed a sensitive and specific method based on surface plasmon resonance (SPR) for detection of insulin autoantibodies (IAA) in serum samples from individuals at high risk of developing type 1 diabetes (T1D). When measuring trace molecules in undiluted sera with label-free techniques like SPR, non-specific adsorption of matrix proteins to the sensor surface is often a problem, since it causes a signal that masks the analyte response. The developed method is an indirect competitive immunoassay designed to overcome these problems. Today, IAA is mainly measured in radio immunoassays (RIAs), which are time consuming and require radioactively labeled antigen. With our SPR-based immunoassay the overall assay time is reduced by a factor of >100 (4 days to 50 min), while sensitivity is maintained at a level comparable to that offered by RIA.
Place, publisher, year, edition, pages
2008. Vol. 24, no 4, 876-881 p.
SPR, Type 1 diabetes, Insulin autoantibodies, Indirect competitive immunoassay, RIA
National CategoryOther Basic Medicine
IdentifiersURN: urn:nbn:se:liu:diva-12469DOI: 10.1016/j.bios.2008.07.018OAI: oai:DiVA.org:liu-12469DiVA: diva2:181
The status of article IV on the day of defence was: Accepted.2008-09-062008-09-062011-01-28Bibliographically approved