Influence Of Optical Properties and Fiber separation on Laser Doppler Flowmetry
2002 (English)In: Journal of Biomedical Optics, ISSN 1083-3668, Vol. 7, no 2, 236-243 p.Article in journal (Refereed) Published
Microcirculatory blood flow can be measured using a laser Doppler flowmetry (LDF) probe. However, the readings are affected by tissue optical properties (absorption and scattering coefficient; µa and µs) and probe geometry. In this study the influence of optical properties (µa∈[0.053, 0.23] mm-1; µs∈[14.7, 45.7] mm-1) on LDF perfusion and sampling depth were evaluated for different fiber separations. In-vitro measurements were made on a sophisticated tissue phantom with known optical properties, mimicking blood flow at different depths. Monte Carlo simulations were carried out to extend the geometry of the tissue phantom.
A good correlation between measured and simulated data was found. The
simulations showed that, for a fixed flow at a discrete depth, the influence of µs or µa on the LDF perfusion increased with increasing flow depth and decreased with increasing fiber separation. For a homogeneous flow distribution, however, the perfusion varied 40% due to the variations in optical properties, almost independent of fiber separation (0.23-1.61 mm). Therefore, the effect in real tissue is likely to vary due to the unknown heterogeneous blood flow distribution. Further, LDF sampling depth increased with decreasing µs or µa and increasing fiber separation. For a fiber separation of 0.46 mm, the e-1 sampling depth ranged from 0.21-0.39 mm.
Place, publisher, year, edition, pages
Journal of Biomedical Optics , 2002. Vol. 7, no 2, 236-243 p.
Doppler effect, fiber optics, laser Doppler flowmetry, Monte Carlo, simulations, optical properties, sampling depth
Engineering and Technology
IdentifiersURN: urn:nbn:se:liu:diva-11717DOI: 10.1117/1.1463049OAI: oai:DiVA.org:liu-11717DiVA: diva2:18130
Marcus Larsson, Wiendelt Steenbergen and Tomas Strömberg, Influence Of Optical Properties and Fiberseparation on Laser Doppler Flowmetry, 2002, Journal of Biomedical Optics, (7), 236-243. http://spiedigitallibrary.aip.org/journals/doc/JBOPFO-ft/vol_7/iss_2/236_1.html Copyright 2002 Society of Photo-Optical Instrumentation Engineers. This paper was published in Journal of Biomedical Optics and is made available as an electronic reprint with permission of SPIE. One print or electronic copy may be made for personal use only. Systematic or multiple reproduction, distribution to multiple locations via electronic or other means, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper are prohibited.2002-05-022002-05-022016-08-31