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The periodontal pathogen Porphyromonas gingivalis cleaves apoB-100 and increases the expression of apoM in LDL in whole blood leading to cell proliferation
Linköping University, Department of Medical and Health Sciences, Pharmacology. Linköping University, Faculty of Health Sciences.
Linköping University, Department of Clinical and Experimental Medicine. Linköping University, Faculty of Health Sciences.
Östergötlands Läns Landsting, Centre for Medicine, Occupational and Environmental Medicine Centre. Linköping University, Department of Medical and Health Sciences. Linköping University, Faculty of Health Sciences.
Linköping University, Department of Medical and Health Sciences, Pharmacology. Linköping University, Faculty of Health Sciences.
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2008 (English)In: Journal of Internal Medicine, ISSN 0954-6820, E-ISSN 1365-2796, Vol. 263, no 5, 558-571 p.Article in journal (Refereed) Published
Abstract [en]

Objective: Several studies support an association between periodontal disease and atherosclerosis with a crucial role for the pathogen Porphyromonas gingivalis. This study aims to investigate the proteolytic and oxidative activity of P. gingivalis on LDL in a whole blood system by using a proteomic approach and analyze the effects of P. gingivalis-modifed LDL on cell proliferation.

Methods: The cellular effects of P. gingivalis in human whole blood were assessed using lumi-aggregometry analyzing reactive oxygen species (ROS) production and aggregation. Blood was incubated for 30 min with P. gingivalis, whereafter LDL was isolated and a proteomic approach was applied to examine protein expression. LDL-oxidation was determined by analyzing the formation of protein carbonyls. The effects of P. gingivalis-modifed LDL on fibroblast proliferation were studied using the MTS-assay.

Results: Incubation of whole blood with P. gingivalis caused an extensive aggregation and ROS-production, indicating platelet and leukocyte activation. LDL prepared from the bacteria-exposed blood showed an increased protein oxidation, elevated levels of apoM and formation of two apoB-100 N-terminal fragments. P. gingivalis-modified LDL markedly increased the growth of fibroblasts. Inhibition of gingipain R suppressed the modification of LDL by P. gingivalis.

Conclusions: The ability of P. gingivalis to change the protein expression and the proliferative capacity of LDL may represent a crucial event in periodontitis-associated atherosclerosis.

Place, publisher, year, edition, pages
Institutionen för medicin och hälsa , 2008. Vol. 263, no 5, 558-571 p.
Keyword [en]
atherosclerosis, growth factors, infection, inflammation, lipoproteins
National Category
Medical and Health Sciences
Identifiers
URN: urn:nbn:se:liu:diva-11870DOI: 10.1111/j.1365-2796.2007.01917.xOAI: oai:DiVA.org:liu-11870DiVA: diva2:18274
Note

The definitive version is available at www.blackwell-synergy.com: Torbjörn Bengtsson, Helen Karlsson, Patrik Gunnarsson, Caroline Skoglund, Charlotte Elison, Per Leanderson and Mats Lindahl, The periodontal pathogen Porphyromonas gingivalis cleaves apoB-100 and increases the expression of apoM in LDL in whole blood leading to cell proliferation, 2008, Journal of Internal Medicine, (263), 5, 558-571. http://dx.doi.org/10.1111/j.1365-2796.2007.01917.x. Copyright: Blackwell Publishing www.blackwell-synergy.com

Available from: 2008-05-21 Created: 2008-05-21 Last updated: 2017-12-13Bibliographically approved

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Bengtsson, TorbjörnKarlsson, HelenGunnarsson, PatrikSkoglund, CarolineElison, CharlotteLeanderson, PerLindahl, Mats

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Bengtsson, TorbjörnKarlsson, HelenGunnarsson, PatrikSkoglund, CarolineElison, CharlotteLeanderson, PerLindahl, Mats
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PharmacologyFaculty of Health SciencesDepartment of Clinical and Experimental MedicineOccupational and Environmental Medicine CentreDepartment of Medical and Health SciencesOccupational and Environmental Medicine
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