Analysis of genetic relatedness using DNA microarrays
Independent thesis Advanced level (degree of Master (Two Years)), 20 credits / 30 HE creditsStudent thesis
Analysis of genetic relatedness is of great importance in forensic casework such as immigration and identification cases. The conventional methods for relationship testing are not sufficient in the most complicated cases, because more genetic markers are required to obtain results with satisfactory statistical security. This study demonstrates that microarrays, which can be used to genotype thousands of single nucleotide polymorphisms (SNPs), could be a promising solution to this problem. The microarray technique used in this study performed very well on blood samples and also worked well in combination with whole genome amplification, but did not generate any results when used on severely degraded materials.
Markers suitable for relatedness analysis were selected from the microarray and were successfully tested on families with known genetic relations. Although a maximum of 64 autosomal markers were used, there is a great potential of selecting the hundreds or thousands of markers that may be required in some cases of relatedness investigation.
Place, publisher, year, edition, pages
2009. , 65 p.
Microarray, DNA chip, Single nucleotide polymorphism, Genetic relatedness, Linkage
Industrial Biotechnology Other Industrial Biotechnology
IdentifiersURN: urn:nbn:se:liu:diva-16490ISRN: LiU-IKE-EX—09/03OAI: oai:DiVA.org:liu-16490DiVA: diva2:200825
2009-01-22, Karljohan, Hälsouniversitetet, 581 83, Linköping, 10:00 (Swedish)
Holmlund, Gunilla, Ph.D
Lindgren, Per-Eric, Ph.D, Associate professor