liu.seSearch for publications in DiVA
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • oxford
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Enrichment strategy development for phosphoproteome analysis of saccharomyces cerevisiae
2009 (English)Independent thesis Advanced level (degree of Master (Two Years)), 20 credits / 30 HE creditsStudent thesis
Abstract [en]

The reversible phosphorylation of proteins is central to regulating most aspects of cell function. Malfunction in this critical cellular process have been implicated to cause diseases such as diabetes, cancer, and Alzheimer’s. Recent advances in mass spectrometry have made it possible to study this important post translational modification on a proteome-wide scale. However, to be able to do so, enrichment of phosphorylated peptides is required. Pairwise comparison of individual steps in an enrichment procedure and simultaneous improvement of data analysis resulted in a protocol which allowed high confidence identification of 2,131 unique phosphorylated peptides from 1,026 proteins. Thereby not only establishing a working protocol for phosphopeptide enrichment in the Griffin Lab, but also generating the largest list of proteins phosphorylated under normal conditions in yeast to date.

Place, publisher, year, edition, pages
2009. , 36 p.
Keyword [en]
Proteomics, mass spectrometry, phosphorylation, yeast, IMAC, SCX, Sequest
National Category
Other Industrial Biotechnology
Identifiers
URN: urn:nbn:se:liu:diva-16997ISRN: LITH-IFM-A-EX--09/2040--SEOAI: oai:DiVA.org:liu-16997DiVA: diva2:200980
Presentation
6-135 Jackson Hall, Minneapolis (English)
Uppsok
fysik/kemi/matematik
Supervisors
Examiners
Available from: 2009-03-03 Created: 2009-03-02 Last updated: 2009-03-03Bibliographically approved

Open Access in DiVA

fulltext(2156 kB)488 downloads
File information
File name FULLTEXT01.pdfFile size 2156 kBChecksum SHA-512
e97d0e05370b5213f60410b2b31f666a3c1459af3601661e5a432304d36d5d81fd96aed61152089ff6a6ff390f9b65f80fb90d167b71943634097f6574538019
Type fulltextMimetype application/pdf

Search in DiVA

By author/editor
Lundemo, Pontus
Other Industrial Biotechnology

Search outside of DiVA

GoogleGoogle Scholar
Total: 488 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

urn-nbn

Altmetric score

urn-nbn
Total: 159 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • oxford
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf