liu.seSearch for publications in DiVA
Change search
ReferencesLink to record
Permanent link

Direct link
Mitochondrial DNA Damage in Iron Overload
University of Louisville.
University of Louisville.
University of Louisville.
Linköping University, Department of Clinical and Experimental Medicine, Oncology . Linköping University, Faculty of Health Sciences. Östergötlands Läns Landsting, Centre of Surgery and Oncology, Department of Oncology UHL.
Show others and affiliations
2009 (English)In: JOURNAL OF BIOLOGICAL CHEMISTRY, ISSN 0021-9258, Vol. 284, no 8, 4767-4775 p.Article in journal (Refereed) Published
Abstract [en]

Chronic iron overload has slow and insidious effects on heart, liver, and other organs. Because iron-driven oxidation of most biologic materials (such as lipids and proteins) is readily repaired, this slow progression of organ damage implies some kind of biological "memory." We hypothesized that cumulative iron-catalyzed oxidant damage to mtDNA might occur in iron overload, perhaps explaining the often lethal cardiac dysfunction. Real time PCR was used to examine the " intactness" of mttDNA in cultured H9c2 rat cardiac myocytes. After 3 -5 days exposure to high iron, these cells exhibited damage to mtDNA reflected by diminished amounts of near full-length 15.9-kb PCR product with no change in the amounts of a 16.1-kb product from a nuclear gene. With the loss of intact mtDNA, cellular respiration declined and mRNAs for three electron transport chain subunits and 16 S rRNA encoded by mtDNA decreased, whereas no decrements were found in four subunits encoded by nuclear DNA. To examine the importance of the interactions of iron with metabolically generated reactive oxygen species, we compared the toxic effects of iron in wild-type and rhoo cells. In wild-type cells, elevated iron caused increased production of reactive oxygen species, cytostasis, and cell death, whereas the rhoo cells were unaffected. We conclude that long-term damage to cells and organs in iron-overload disorders involves interactions between iron and mitochondrial reactive oxygen species resulting in cumulative damage to mtDNA, impaired synthesis of respiratory chain subunits, and respiratory dysfunction.

Place, publisher, year, edition, pages
2009. Vol. 284, no 8, 4767-4775 p.
National Category
Medical and Health Sciences
URN: urn:nbn:se:liu:diva-17151DOI: 10.1074/jbc.M806235200OAI: diva2:202141
Original Publication:Xueshan Gao, Jian Li Campian, Mingwei Qian, Xiao-Feng Sun and John Wallace Eaton, Mitochondrial DNA Damage in Iron Overload, 2009, JOURNAL OF BIOLOGICAL CHEMISTRY, (284), 8, 4767-4775. American Society for Biochemistry and Molecular Biology from: 2009-03-12 Created: 2009-03-07 Last updated: 2009-08-18Bibliographically approved

Open Access in DiVA

fulltext(455 kB)504 downloads
File information
File name FULLTEXT01.pdfFile size 455 kBChecksum SHA-512
Type fulltextMimetype application/pdf

Other links

Publisher's full text

Search in DiVA

By author/editor
Sun, Xiao-FengWallace Eaton, John
By organisation
Oncology Faculty of Health SciencesDepartment of Oncology UHLExperimental Pathology Department of Clinical Pathology and Clinical Genetics
Medical and Health Sciences

Search outside of DiVA

GoogleGoogle Scholar
Total: 504 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 114 hits
ReferencesLink to record
Permanent link

Direct link