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Collagen-phosphorylcholine interpenetrating network hydrogels as corneal substitutes
University of Ottawa.
University of Ottawa.
University of Ottawa.
Linköping University, Department of Clinical and Experimental Medicine, Ophthalmology. Linköping University, Faculty of Health Sciences. Östergötlands Läns Landsting, Reconstruction Centre, Department of Ophthalmology UHL/MH.
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2009 (English)In: BIOMATERIALS, ISSN 0142-9612, Vol. 30, no 8, 1551-1559 p.Article in journal (Refereed) Published
Abstract [en]

A biointeractive collagen-phospholipid corneal Substitute was fabricated from interpenetrating polymeric networks comprising 1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide and N-hydroxysuccinimide crosslinked porcine atelocollagen, and poly(ethylene glycol) diacrylate crosslinked 2-methacryloyloxyethyl phosphorylcholine (MPC). The resulting hydrogels showed ail overall increase in mechanical strength beyond that of either original component and enhanced stability against enzymatic digestion (by collagenase) or UV degradation. More strikingly, these hydrogels retained the full biointeractive, cell friendly properties of collagen in promoting corneal cell and nerve in-growth and, regeneration (despite MPCs known anti-adhesive properties). Measurements of refractive indices, white light transmission and backscatter showed the optical properties of collagen-MPC are comparable or superior to those of the human cornea.In addition, the glucose and albumin permeability were comparable to those Of human corneas. Twelve-month post-implantation results of collagen-MPC hydrogels into mini-pigs showed regeneration of corneal tissue (epithelium, stroma) as well as the tear film and sensory nerves. We also show that porcine collagen can be Substituted with recombinant human collagen, resulting in a fully-synthetic implant that is free from the potential risks of disease transmission (e.g. prions) present in animal Source materials.

Place, publisher, year, edition, pages
2009. Vol. 30, no 8, 1551-1559 p.
Keyword [en]
Recombinant collagen, Phospholipid, Cornea substitute, Tissue engineering, Biomedical regeneration
National Category
Medical and Health Sciences
URN: urn:nbn:se:liu:diva-17164DOI: 10.1016/j.biomaterials.2008.11.022OAI: diva2:202153
Available from: 2009-03-07 Created: 2009-03-07 Last updated: 2013-12-17

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Fagerholm, PerLagali, Neil SGriffith, May
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OphthalmologyFaculty of Health SciencesDepartment of Ophthalmology UHL/MH
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