Effects of PDGF-C and PDGF-D on monocyte migration and MMP-2 and MMP-9 expression
2009 (English)In: Atherosclerosis, ISSN 0021-9150, Vol. 202, no 2, 415-423 p.Article in journal (Refereed) Published
Background and nuns: Atherosclerosis is a chronic inflammatory process involving the activity of several cytokines and growth factors. Platelet-derived growth factor-A (PDGF-A) and PDGF-B are important mitogens and chemoattractants for monocytcs as well as smooth muscle cells. We sought to identify the role of PDGF-C and PDGF-D, two new members of the PDGF family, in monocyte migration and differentiation. We also assessed their effects in regulating matrix metalloproteinase-2 (MMP-2) and MMP-9. which are important for cell migration.
Methods and results: PDGF-C and PDGF-D were expressed in macrophages, smooth muscle cells, and endothelial cells in human atherosclerotic plaques, as shown by immunohistochemical analysis. PDGF-C and PDGF-I) mRNA and protein expression was induced after differentiation of THP-1 monocytes to macrophages, and both PDGF-C and PDGF-D induced MMP-9 mRNA expression in a concentration-dependent manner. Treatment of cells with PDGF-C or PDGF-D enhanced the secretion of MMP-2 and MMP-9 in a cell-dependent manner. In a migration assay using a Boyden chamber with 8 mu m pore size, PDGF-C and PDGF-D attracted THP-1 monocytes in a concentration-dependent manner.
Conclusions: Our data suggest that PDGF-C and PDGF-D, like PDGF-A and PDGF-B, play important roles in atherosclerosis by stimulating MMP activity and influencing monocyte migration.
Place, publisher, year, edition, pages
2009. Vol. 202, no 2, 415-423 p.
Protease, Growth factor, Extracellular matrix, Atherosclerosis
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:liu:diva-17166DOI: 10.1016/j.atherosclerosis.2008.04.050OAI: oai:DiVA.org:liu-17166DiVA: diva2:202155