A short estrogen-responsive N-terminal galanin homologue found in rat brain and gut with antiserum raised against rat galanin(1-16)
2007 (English)In: Neurochemical Research, ISSN 0364-3190 (Print) 1573-6903 (Online), Vol. 31, no 2, 177-188 p.Article in journal (Refereed) Published
Galanin-like peptide (GALP) is currently the only known galanin(1-29) homologue. However, three different galanin receptors, of which GalR3 exhibits comparatively low affinity for galanin(1-29), and molecular heterogeneity of immunoreactive galanin are arguments for presence of other endogenous galanin homologues. Since antibodies recognize three-dimensional structures of 3–5 amino acids in a peptide, we raised antibodies in rabbits against galanin(1-16) conjugated to bovine serum albumin, looking for the presence of endogenous N-terminal galanin homologues in rat tissues. The antiserum selected had 7,830 times higher avidity for galanin(1-16) compared to galanin(1-29). A single immunoreactive component with a Stokes radius of about 8 amino acids was found. Immunohistochemistry strongly suggested that this immunoreactivity is localised in the same neurons as galanin(1-29). Furthermore, its concentration was increased in response to estrogen treatment in the same brain regions as galanin(1-29), although not as rapidly. The present results indicate the presence of a novel endogenous N-terminal galanin homologue.
Place, publisher, year, edition, pages
2007. Vol. 31, no 2, 177-188 p.
Hippocampus, HPLC, Hypothalamus, Immunohistochemistry, Radioimmunoassay
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:liu:diva-13382DOI: 10.1007/s11064-005-9007-5OAI: oai:DiVA.org:liu-13382DiVA: diva2:20545