liu.seSearch for publications in DiVA
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • oxford
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Elucidation of the product synthesis of the sesquiterpene synthase Cop6 isolated from Coprinus cinereus
Linköping University, Department of Physics, Chemistry and Biology.
2009 (English)Independent thesis Advanced level (degree of Master (Two Years)), 20 credits / 30 HE creditsStudent thesis
Abstract [en]

Mushrooms are believed to have a great potential for production of bioactive metabolites e. g. terpenes, a group of interesting compounds with diverse chemical properties such as antitumour and antibacterial activity. Cop6 is a terpene cyclase isolated from the mushroom Coprinus cinereus that catalyzes the cyclization of farnesyl diphosphate (FPP) to mainly α-cuprenene. In this study gas chromatography combined with mass spectroscopy (GC-MS) is used to analyze the product profile of Cop6 mutants created by PCR based site directed mutagenesis. The goal is to produce trichodiene, the parent hydrocarbon in the biosynthesis of trichothecene antibiotics and mycotoxins. Valine instead of tyrosine in amino acid position 195 resulted in cyclisation of (E)-β-Farnesene and (3Z,6E)-α-Farnesene besides the products of the wild type enzyme. Another mutant with aspartic acid instead of asparagine in position 224 resulted in the synthesis of β-Bisabolene except for α-cuprenene and methionine in position 74 instead of isoleucine killed the activity of the cyclase. Furthermore, an attempt to saturation of position 98 was made, resulting in four mutants. Two of them essentially killed the activity of the cyclase whereas two had minor effect of the product profile compared to the wild type. 

Place, publisher, year, edition, pages
2009. , 36 p.
Keyword [en]
Site directed mutagenesis, PCR, Gas Chromatography-Mass Spectrometry, sesquiterpenes, Coprinus cinereus
National Category
Biochemistry and Molecular Biology
Identifiers
URN: urn:nbn:se:liu:diva-17252ISRN: LITH-IFM-A-EX--09/2039--SEOAI: oai:DiVA.org:liu-17252DiVA: diva2:207778
Presentation
6-135 Jackson Hall, 321 Church St. SE, Minneapolis (English)
Uppsok
fysik/kemi/matematik
Supervisors
Examiners
Available from: 2009-03-16 Created: 2009-03-12 Last updated: 2009-03-16Bibliographically approved

Open Access in DiVA

fulltext(928 kB)424 downloads
File information
File name FULLTEXT01.pdfFile size 928 kBChecksum SHA-512
0875ac9c4700561f420461d6df41c80238ab3940c87491a2a2e1a2aef32b4068d4118c60c805710f4145297c210119935f62049b49f0f4344ba7b7b0689b568a
Type fulltextMimetype application/pdf

By organisation
Department of Physics, Chemistry and Biology
Biochemistry and Molecular Biology

Search outside of DiVA

GoogleGoogle Scholar
Total: 424 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

urn-nbn

Altmetric score

urn-nbn
Total: 378 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • oxford
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf