Solid-phase extraction with end-capped C2 columns for the routine measurement of racemic citalopram and metabolites in plasma by high-performance liquid chromatography
1997 (English)In: Journal of Chromatography B: Biomedical Sciences and Applications, ISSN 1570-0232, Vol. 702, no 1-2, 234-239 p.Article in journal (Refereed) Published
An assay based on solid-phase extraction followed by high-performance liquid chromatography (HPLC) was developed for the measurement of citalopram and its main metabolites desmethylcitalopram and didesmethylcitalopram. The best extraction procedure was performed with end-capped C2 column utilising secondary silanol interactions to obtain clean extract. The HPLC analysis was done on a phenyl column with a mobile phase without any amine additives. Fluorescence detection gave a limit of detection of 0.8 nmol/l plasma for the compounds analysed.
Place, publisher, year, edition, pages
1997. Vol. 702, no 1-2, 234-239 p.
Citalopram, Desmethylcitalopram, Didesmethylcitalopram
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:liu:diva-13689DOI: 10.1016/S0378-4347(97)00366-6OAI: oai:DiVA.org:liu-13689DiVA: diva2:21164