A cell-based sensor system for toxicity testing using multiwavelength fluorescence spectroscopy
2009 (English)In: ANALYTICAL BIOCHEMISTRY, ISSN 0003-2697, Vol. 387, no 2, 271-275 p.Article in journal (Refereed) Published
A novel cell-based fluorometric sensor system for toxicity monitoring is described, which uses functional spontaneously contracting cardiomyocytes (HL-1 cell line) as the biological recognition element. Based on these highly specialized cells, it has the potential of providing a sensitive and relevant analytical in vitro toxicity testing method. The system was configured by propagating the surface-attaching HL-1 cardiomyocytes in the wells of a 96-well microtiter plate and connecting the plate via an optical fiber to a fluorescence spectrometer capable of excitation-emission matrix scanning. The fluorescence data were analyzed using a conventional spectral analysis software program. The performance of the system for detection of general cytotoxicity to the cells was evaluated using three well-known drugs: verapamil, quinidine, and acetaminophen. The dose-response curves were assessed and the EC50 values were determined (0.10 +/- 0.007, 0.23 +/- 0.025, and 12.32 +/- 2.40 mM, respectively). Comparison with in vitro and in vivo reference data for the drugs showed good correlations, suggesting that this cell-based sensor system Could be a useful tool in pharmacological in vitro drug testing.
Place, publisher, year, edition, pages
2009. Vol. 387, no 2, 271-275 p.
Autofluorescence, HL-1 cell line, Cardiomyocytes, Cytotoxicity screening, Drug testing, Microtiter plate, Tryptophan fluorescence, In vitro toxicity
Engineering and Technology
IdentifiersURN: urn:nbn:se:liu:diva-17736DOI: 10.1016/j.ab.2009.01.027OAI: oai:DiVA.org:liu-17736DiVA: diva2:211739