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A cell-based sensor system for toxicity testing using multiwavelength fluorescence spectroscopy
Linköping University, Department of Physics, Chemistry and Biology, Biotechnology. Linköping University, Faculty of Science & Engineering.
Linköping University, Department of Physics, Chemistry and Biology, Biotechnology. Linköping University, Faculty of Science & Engineering.
Linköping University, Department of Physics, Chemistry and Biology, Biotechnology. Linköping University, The Institute of Technology.
Linköping University, Department of Physics, Chemistry and Biology, Biotechnology. Linköping University, The Institute of Technology.
2009 (English)In: ANALYTICAL BIOCHEMISTRY, ISSN 0003-2697, Vol. 387, no 2, 271-275 p.Article in journal (Refereed) Published
Abstract [en]

A novel cell-based fluorometric sensor system for toxicity monitoring is described, which uses functional spontaneously contracting cardiomyocytes (HL-1 cell line) as the biological recognition element. Based on these highly specialized cells, it has the potential of providing a sensitive and relevant analytical in vitro toxicity testing method. The system was configured by propagating the surface-attaching HL-1 cardiomyocytes in the wells of a 96-well microtiter plate and connecting the plate via an optical fiber to a fluorescence spectrometer capable of excitation-emission matrix scanning. The fluorescence data were analyzed using a conventional spectral analysis software program. The performance of the system for detection of general cytotoxicity to the cells was evaluated using three well-known drugs: verapamil, quinidine, and acetaminophen. The dose-response curves were assessed and the EC50 values were determined (0.10 +/- 0.007, 0.23 +/- 0.025, and 12.32 +/- 2.40 mM, respectively). Comparison with in vitro and in vivo reference data for the drugs showed good correlations, suggesting that this cell-based sensor system Could be a useful tool in pharmacological in vitro drug testing.

Place, publisher, year, edition, pages
2009. Vol. 387, no 2, 271-275 p.
Keyword [en]
Autofluorescence, HL-1 cell line, Cardiomyocytes, Cytotoxicity screening, Drug testing, Microtiter plate, Tryptophan fluorescence, In vitro toxicity
National Category
Engineering and Technology
Identifiers
URN: urn:nbn:se:liu:diva-17736DOI: 10.1016/j.ab.2009.01.027OAI: oai:DiVA.org:liu-17736DiVA: diva2:211739
Available from: 2009-04-17 Created: 2009-04-17 Last updated: 2015-05-28

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Fritzsche, MichaelFredriksson, MagnusCarlsson, MariaMandenius, Carl-Fredrik

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