Cell–cell adherence as a selection method for the generation of anti-melanoma monoclonal antibodies
1997 (English)In: Journal of Immunological Methods, ISSN 0022-1759, Vol. 203, no 1, 103-109 p.Article in journal (Refereed) Published
The aim of the present study was to obtain monoclonal antibodies (mAbs) recognising human melanoma-associated antigens after immunisation of BALB/c mice with a 70–150 kDa membrane fraction from melanoma tumour tissues. Screening of specific antibody- producing hybridomas was performed using a novel cell–cell adherence method with the melanoma cell line M-14. Three mAbs of IgG1 isotype were selected: Mel-1, Mel-2 and Mel-3 which recognised the immunogen by ELISA and stained several melanoma cell lines positive in immunofluorescence. The molecular weight of the antigen was studied by different methods; a 170-kDa band was identified following immunoblotting of tumour lysate and a 72-kDa band was observed following immunoaffinity purification. Cell–cell adherence appears to be a reliable procedure for the generation of mAbs against native cellular antigens.
Place, publisher, year, edition, pages
1997. Vol. 203, no 1, 103-109 p.
Malignant melanoma; Hybridoma selection; Monoclonal antibody
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:liu:diva-13713DOI: 10.1016/S0022-1759(97)00025-2OAI: oai:DiVA.org:liu-13713DiVA: diva2:21195