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Overland flow systems for treatment of landfill leachates: Potential nitrification and structure of the ammonia-oxidising bacterial community during a growing season
Linköping University, Department of Clinical and Experimental Medicine, Medical Microbiology . Linköping University, Faculty of Health Sciences.
Linköping University, Department of Physics, Chemistry and Biology. Linköping University, The Institute of Technology.
Linköping University, Department of Physics, Chemistry and Biology. Linköping University, The Institute of Technology.
Linköping University, Department of Clinical and Experimental Medicine, Medical Microbiology . Linköping University, Faculty of Health Sciences.
2007 (English)In: Soil Biology and Biochemistry, ISSN 0038-0717, E-ISSN 1879-3428, Vol. 39, no 1, 127-138 p.Article in journal (Refereed) Published
Abstract [en]

Overland flow systems are useful for treating landfill leachates, because they provide favourable conditions for nitrification and they are easy to maintain. However, little is known about the microbial communities in such systems or the nitrification capacity of those microorganisms. In this study, seasonal variations in potential nitrification and in community composition of nitrifying bacteria were investigated in two overland flow areas receiving leachate from landfills at Korslöt and Hagby, Sweden. Samples were collected in the settling ponds sediment and at two depths in the overland flow areas (the macrophyte litter layer and the rhizosphere) in May, August and November 2003. A short-term incubation method was used to measure potential oxidation of ammonia and nitrite (designated PAO and PNO). The ammonia-oxidising bacterial (AOB) community was investigated using a 16S rRNA gene approach that included PCR amplification and analysis of PCR products by denaturing gradient gel electrophoresis (DGGE), followed by nucleotide sequencing and phylogenetic analysis.

PAO was determined in the range 5–2700 (NO2−+NO3−)-N g−1 dw d−1 and PNO in the range 60–2000 μg NO2−-N g−1 dw d−1. At Korslöt, PAO and PNO showed similar temporal variation in the different ecosystems, whereas no such relationship was noticed at Hagby. Considering both sites, there was no obvious change in the composition of the AOB community over the growing season. However, the composition did differ between the ecosystems: Nitrosomonas-like sequences were more common in the ponds, and in the litter layers they were found as often as Nitrosospira-like sequences, whereas Nitrosospira-like sequences were more common in the rhizospheres. Altogether, we found nine different AOB sequences, five Nitrosomonas-like and four Nitrosospira-like, which belonged to clusters 0, 2, 3b, 6a, 6b and 7. There was no apparent relationship between the number of AOB populations and the PAO in different soil layers and sediments.

Place, publisher, year, edition, pages
Elesevire , 2007. Vol. 39, no 1, 127-138 p.
Keyword [en]
Ammonia-oxidising bacterial community; Landfill leachates; Nitrification; Overland flow; 16S rDNA; Denaturing gradient gel electrophoresis
National Category
Medical and Health Sciences
Identifiers
URN: urn:nbn:se:liu:diva-17919DOI: 10.1016/j.soilbio.2006.06.016OAI: oai:DiVA.org:liu-17919DiVA: diva2:212887
Available from: 2009-04-24 Created: 2009-04-24 Last updated: 2017-12-13Bibliographically approved
In thesis
1. Nitrifyers in constructed wetlands treating landfill leachates
Open this publication in new window or tab >>Nitrifyers in constructed wetlands treating landfill leachates
2008 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Landfill leachate is produced many years after a landfill site closes. Hence, treatment by “natural methods”, as e.g. constructed wetlands, with low management requirements is attractive. Constructed wetlands usually provide both shallow and deep areas with aerobic and anaerobic zones, which is suitable for nitrification followed by denitrification of the ammonium-rich leachates. Full-scale treatment systems are influenced by climatic variables that affect the microbial community. Also, the operational strategy can have a considerable impact on both activity and composition of the microorganisms. Many studies have measured inflow/outflow water quality in treatment systems. Such “black box” studies describe the treatment efficiency but add little to an increased understanding of theorganisms performing the treatment or the spatial distribution of their activities and treatment processes.

In this thesis we investigated seasonal and annual changes in potential nitrification and denitrification, and in the corresponding bacterial communities in constructed wetlands treating landfill leachates. Variations in the potential activity in full-scale systems were investigated over several years, using short-term incubation. The composition of the bacterial communities was investigated using a group-specific PCR primer pair targeting the 16S rRNA genes or a primer pair targeting the funtional gene nosZ. The PCR products were analysed by denaturing gradient gelelectrophoresis and subsequent nucleotide sequencing and phylogentic analysis.

A stable ammonia-oxidising bacterial (AOB) community composition and potential ammonia-oxidation (PAO) were detected in the system with a year-round operation. On the other hand, changes in the AOB community composition which followed the operational schedule were detected in the overland flow area (OFA) running seasonally. Furthermore, the influence of operational strategy was indicated by a low PAO in the wastewater overland flow area and compact constructed wetland receiving high hydraulic loads, indicating the value of aeration. Higher PAO was detected in the OFAs where the hydraulic load followed literature guidelines.

All systems supported diverse AOB communities, represented by several Nitrosomonas and Nitrosospira populations. The number of different populations detected in these wetlands was much higher than reported in municipal wastewater treatment plants, and differed from those in a parallel OFA treating municipal wastewater. Furthermore, the large variation in both potential activity and sequences detected in replicate samples suggests that such systems are spatially heterogenic.

Place, publisher, year, edition, pages
Linköping: Linköping University Electronic Press, 2008. 47 p.
Series
Linköping University Medical Dissertations, ISSN 0345-0082 ; 1067
National Category
Medical and Health Sciences
Identifiers
urn:nbn:se:liu:diva-17923 (URN)978-91-7393-872-3 (ISBN)
Public defence
2008-06-04, Aulan, Hälsans hus, Campus US, Linköpings Universitet , Linköping, 14:00 (English)
Opponent
Supervisors
Available from: 2009-04-24 Created: 2009-04-24 Last updated: 2009-04-24Bibliographically approved

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Sundberg, CarinaStendahl, Jenny S. K. Sundblad-Tonderski, Karin Lindgren, Per-Eric

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