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Potential nitrification and denitrification and the corresponding composition of the bacterial communities in a compact constructed wetland treating landfill leachates
Linköping University, Department of Clinical and Experimental Medicine, Medical Microbiology . Linköping University, Faculty of Health Sciences.
Linköping University, Department of Physics, Chemistry and Biology. Linköping University, The Institute of Technology.
Linköping University, Department of Clinical and Experimental Medicine, Medical Microbiology . Linköping University, Faculty of Health Sciences.
2007 (English)In: Water Science and Technology, ISSN 0273-1223, Vol. 56, no 3, 159-166 p.Article in journal (Refereed) Published
Abstract [en]

Constructed wetlands can be used to decrease the high ammonium concentrations in landfill leachates. We investigated nitrification/denitrification activity and the corresponding bacterial communities in landfill leachate that was treated in a compact constructed wetland, Tveta Recycling Facility, Sweden. Samples were collected at three depths in a filter bed and the sediment from a connected open pond in July, September and November 2004. Potential ammonia oxidation was measured by short-term incubation method and potential denitrification by the acetylene inhibition technique. The ammonia-oxidising and the denitrifying bacterial communities were investigated using group-specific PCR primers targeting 16S rRNA genes and the functional gene nosZ, respectively. PCR products were analysed by denaturing gradient gel electrophoresis and nucleotide sequencing. The same degree of nitrification activity was observed in the pond sediment and at all levels in the filter bed, whereas the denitrification activity decreased with filter bed depth. Denitrification rates were higher in the open pond, even though the denitrifying bacterial community was more diverse in the filter bed. The ammonia-oxidising community was also more varied in the filter bed. In the filter bed and the open pond, there was no obvious relationship between the nitrification/denitrification activities and the composition of the corresponding bacterial communities.

Place, publisher, year, edition, pages
2007. Vol. 56, no 3, 159-166 p.
Keyword [en]
Denitrification, DGGE, landfill leachates, nitrification; nosZ, 16S rrNA
National Category
Medical and Health Sciences
Identifiers
URN: urn:nbn:se:liu:diva-17920DOI: 10.2166/wst.2007.524PubMedID: 17802851OAI: oai:DiVA.org:liu-17920DiVA: diva2:212891
Available from: 2009-04-24 Created: 2009-04-24 Last updated: 2011-01-11Bibliographically approved
In thesis
1. Nitrifyers in constructed wetlands treating landfill leachates
Open this publication in new window or tab >>Nitrifyers in constructed wetlands treating landfill leachates
2008 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Landfill leachate is produced many years after a landfill site closes. Hence, treatment by “natural methods”, as e.g. constructed wetlands, with low management requirements is attractive. Constructed wetlands usually provide both shallow and deep areas with aerobic and anaerobic zones, which is suitable for nitrification followed by denitrification of the ammonium-rich leachates. Full-scale treatment systems are influenced by climatic variables that affect the microbial community. Also, the operational strategy can have a considerable impact on both activity and composition of the microorganisms. Many studies have measured inflow/outflow water quality in treatment systems. Such “black box” studies describe the treatment efficiency but add little to an increased understanding of theorganisms performing the treatment or the spatial distribution of their activities and treatment processes.

In this thesis we investigated seasonal and annual changes in potential nitrification and denitrification, and in the corresponding bacterial communities in constructed wetlands treating landfill leachates. Variations in the potential activity in full-scale systems were investigated over several years, using short-term incubation. The composition of the bacterial communities was investigated using a group-specific PCR primer pair targeting the 16S rRNA genes or a primer pair targeting the funtional gene nosZ. The PCR products were analysed by denaturing gradient gelelectrophoresis and subsequent nucleotide sequencing and phylogentic analysis.

A stable ammonia-oxidising bacterial (AOB) community composition and potential ammonia-oxidation (PAO) were detected in the system with a year-round operation. On the other hand, changes in the AOB community composition which followed the operational schedule were detected in the overland flow area (OFA) running seasonally. Furthermore, the influence of operational strategy was indicated by a low PAO in the wastewater overland flow area and compact constructed wetland receiving high hydraulic loads, indicating the value of aeration. Higher PAO was detected in the OFAs where the hydraulic load followed literature guidelines.

All systems supported diverse AOB communities, represented by several Nitrosomonas and Nitrosospira populations. The number of different populations detected in these wetlands was much higher than reported in municipal wastewater treatment plants, and differed from those in a parallel OFA treating municipal wastewater. Furthermore, the large variation in both potential activity and sequences detected in replicate samples suggests that such systems are spatially heterogenic.

Place, publisher, year, edition, pages
Linköping: Linköping University Electronic Press, 2008. 47 p.
Series
Linköping University Medical Dissertations, ISSN 0345-0082 ; 1067
National Category
Medical and Health Sciences
Identifiers
urn:nbn:se:liu:diva-17923 (URN)978-91-7393-872-3 (ISBN)
Public defence
2008-06-04, Aulan, Hälsans hus, Campus US, Linköpings Universitet , Linköping, 14:00 (English)
Opponent
Supervisors
Available from: 2009-04-24 Created: 2009-04-24 Last updated: 2009-04-24Bibliographically approved

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Sundberg, CarinaSundblad-Tonderski, KarinLindgren, Per-Erik

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