Development of the community structure and activity of ammoniaoxidising bacteria in overland flow systems used to treat landfill leachates
2009 (English)In: Journal of Applied Microbiology, ISSN 1364-5072, E-ISSN 1365-2672Article in journal (Other academic) Submitted
Ammonium in landfill leachates can be nitrified in overland flow areas (OFA). We studied OFAs to investigate if changes occur in the ammonium oxidizing community as the ecosystem develops, and the influence of different operating conditions. Samples were collected from the macrophyte litter layers, the rhizospheres and the sediments in their associated settling ponds in May, August, and November during four years. Potential ammonia oxidation (PAO) was investigated by a short-term slurry incubation method. The composition of the ammonia-oxidising bacteria (AOB) communities was investigated by PCR, using a group-specific primer pair, followed by denaturing gradient gel electrophoresis and subsequent sequencing.
A shift from a Nitrosomonas community to a mixture of Nitrosomonas and Nitrosospira was and a gradual increase in PAO was observed, but only in the litter layer in the youngest OFA. Both OFAs had diverse AOB communities belonging to six different clusters. Nitrosomonas clusters predominated in the OFA with higher PAO, whereas Nitrosospira clusters were more common in the OFA with lower PAO. There was a seasonal increase of AOB populations in the OFA that was not in use during winter, and a more stable composition of the AOB community and the PAO in the OFA with year-round application. Keywords: Ammonia-oxidising bacterial community; Landfill leachates; Nitrification; Overland flow; 16S rDNA; Denaturing gradient gel electrophoresis.
Place, publisher, year, edition, pages
Ammonia-oxidising bacterial community, Landfill leachates, Nitrification, Overland flow, 16S rDNA, Denaturing gradient gel electrophoresis
National CategoryMedical and Health Sciences
IdentifiersURN: urn:nbn:se:liu:diva-17921OAI: oai:DiVA.org:liu-17921DiVA: diva2:212892