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Development of the community structure and activity of ammoniaoxidising bacteria in overland flow systems used to treat landfill leachates
Linköping University, Department of Clinical and Experimental Medicine, Medical Microbiology . Linköping University, Faculty of Health Sciences.
Linköping University, Department of Physics, Chemistry and Biology. Linköping University, The Institute of Technology.
Linköping University, Department of Clinical and Experimental Medicine, Medical Microbiology . Linköping University, Faculty of Health Sciences.
2009 (English)In: Journal of Applied Microbiology, ISSN 1364-5072, E-ISSN 1365-2672Article in journal (Other academic) Submitted
Abstract [en]

Ammonium in landfill leachates can be nitrified in overland flow areas (OFA). We studied OFAs to investigate if changes occur in the ammonium oxidizing community as the ecosystem develops, and the influence of different operating conditions. Samples were collected from the macrophyte litter layers, the rhizospheres and the sediments in their associated settling ponds in May, August, and November during four years. Potential ammonia oxidation (PAO) was investigated by a short-term slurry incubation method. The composition of the ammonia-oxidising bacteria (AOB) communities was investigated by PCR, using a group-specific primer pair, followed by denaturing gradient gel electrophoresis and subsequent sequencing.

A shift from a Nitrosomonas community to a mixture of Nitrosomonas and Nitrosospira was and a gradual increase in PAO was observed, but only in the litter layer in the youngest OFA. Both OFAs had diverse AOB communities belonging to six different clusters. Nitrosomonas clusters predominated in the OFA with higher PAO, whereas Nitrosospira clusters were more common in the OFA with lower PAO. There was a seasonal increase of AOB populations in the OFA that was not in use during winter, and a more stable composition of the AOB community and the PAO in the OFA with year-round application. Keywords: Ammonia-oxidising bacterial community; Landfill leachates; Nitrification; Overland flow; 16S rDNA; Denaturing gradient gel electrophoresis.

Place, publisher, year, edition, pages
2009.
Keyword [en]
Ammonia-oxidising bacterial community, Landfill leachates, Nitrification, Overland flow, 16S rDNA, Denaturing gradient gel electrophoresis
National Category
Medical and Health Sciences
Identifiers
URN: urn:nbn:se:liu:diva-17921OAI: oai:DiVA.org:liu-17921DiVA: diva2:212892
Available from: 2009-04-24 Created: 2009-04-24 Last updated: 2010-04-08Bibliographically approved
In thesis
1. Nitrifyers in constructed wetlands treating landfill leachates
Open this publication in new window or tab >>Nitrifyers in constructed wetlands treating landfill leachates
2008 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Landfill leachate is produced many years after a landfill site closes. Hence, treatment by “natural methods”, as e.g. constructed wetlands, with low management requirements is attractive. Constructed wetlands usually provide both shallow and deep areas with aerobic and anaerobic zones, which is suitable for nitrification followed by denitrification of the ammonium-rich leachates. Full-scale treatment systems are influenced by climatic variables that affect the microbial community. Also, the operational strategy can have a considerable impact on both activity and composition of the microorganisms. Many studies have measured inflow/outflow water quality in treatment systems. Such “black box” studies describe the treatment efficiency but add little to an increased understanding of theorganisms performing the treatment or the spatial distribution of their activities and treatment processes.

In this thesis we investigated seasonal and annual changes in potential nitrification and denitrification, and in the corresponding bacterial communities in constructed wetlands treating landfill leachates. Variations in the potential activity in full-scale systems were investigated over several years, using short-term incubation. The composition of the bacterial communities was investigated using a group-specific PCR primer pair targeting the 16S rRNA genes or a primer pair targeting the funtional gene nosZ. The PCR products were analysed by denaturing gradient gelelectrophoresis and subsequent nucleotide sequencing and phylogentic analysis.

A stable ammonia-oxidising bacterial (AOB) community composition and potential ammonia-oxidation (PAO) were detected in the system with a year-round operation. On the other hand, changes in the AOB community composition which followed the operational schedule were detected in the overland flow area (OFA) running seasonally. Furthermore, the influence of operational strategy was indicated by a low PAO in the wastewater overland flow area and compact constructed wetland receiving high hydraulic loads, indicating the value of aeration. Higher PAO was detected in the OFAs where the hydraulic load followed literature guidelines.

All systems supported diverse AOB communities, represented by several Nitrosomonas and Nitrosospira populations. The number of different populations detected in these wetlands was much higher than reported in municipal wastewater treatment plants, and differed from those in a parallel OFA treating municipal wastewater. Furthermore, the large variation in both potential activity and sequences detected in replicate samples suggests that such systems are spatially heterogenic.

Place, publisher, year, edition, pages
Linköping: Linköping University Electronic Press, 2008. 47 p.
Series
Linköping University Medical Dissertations, ISSN 0345-0082 ; 1067
National Category
Medical and Health Sciences
Identifiers
urn:nbn:se:liu:diva-17923 (URN)978-91-7393-872-3 (ISBN)
Public defence
2008-06-04, Aulan, Hälsans hus, Campus US, Linköpings Universitet , Linköping, 14:00 (English)
Opponent
Supervisors
Available from: 2009-04-24 Created: 2009-04-24 Last updated: 2009-04-24Bibliographically approved

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Sundberg, CarinaSundblad-Tonderski, KarinLindgren, Per-Eric

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