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Ammonia oxidation and the corresponding bacterial communities in two overland flow areas treating landfill leachate or wastewater
Linköping University, The Tema Institute, Department of Water and Environmental Studies. Linköping University, Faculty of Arts and Sciences.
Linköping University, Department of Physics, Chemistry and Biology. Linköping University, The Institute of Technology.
Linköping University, Department of Clinical and Experimental Medicine, Medical Microbiology. Linköping University, Faculty of Health Sciences.
2011 (English)In: Overland Flow and Surface Runoff / [ed] Tommy S W Wong, Nova Science Publishers, Inc., 2011, 346- p.Chapter in book (Refereed)
Abstract [en]

A high diversity of ammonium oxidising bacteria (AOB) has been observed in overland flow areas (OFA) treating ammonia-rich landfill leachate. The current section aimed to explore if treatment OFAs in general supports more diverse AOB communities than conventional treatment systems, or if it is a result of effluent composition. The potential ammonium oxidation and the AOB community composition were studied during three seasons in an OFA where one part received wastewater and the other landfill leachate. The AOB communities were investigated using group-specific PCR primers targeting the 16S rRNA gene, and analysed by DGGE and nucleotide sequencing. The potential ammonia oxidation, studied by short-time slurry incubation, was higher in the landfill OFA than in the wastewater area and highest in the litter layer. Higher activity correlated with the appearance of Nitrosomonas sp. belonging to cluster 7. Both overland flow areas supported a more diverse AOB community than in common wastewater treatment plants. Fifteen different AOB sequences were detected, but only three were observed in both OFAs, pointing to the impact of the effluent quality and/or the hydraulic load. The wastewater OFA, which received a higher load of effluents with 5-10 times lower ammonia concentrations, was dominated by AOB populations that are usually found in less favourable conditions.

Place, publisher, year, edition, pages
Nova Science Publishers, Inc., 2011. 346- p.
Keyword [en]
Runoff -- Mathematical models, Streamflow -- Mathematical models, Frictional resistance (Hydrodynamics), ´Water Pollution, Nitrifying bacteria, SCIENCE / Earth Sciences / Hydrology, SCIENCE / Earth Sciences / Hydrology
National Category
Natural Sciences
URN: urn:nbn:se:liu:diva-17922ISBN: 978-1-61122-868-7OAI: diva2:212894
Available from: 2009-04-24 Created: 2009-04-24 Last updated: 2014-12-19Bibliographically approved
In thesis
1. Nitrifyers in constructed wetlands treating landfill leachates
Open this publication in new window or tab >>Nitrifyers in constructed wetlands treating landfill leachates
2008 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Landfill leachate is produced many years after a landfill site closes. Hence, treatment by “natural methods”, as e.g. constructed wetlands, with low management requirements is attractive. Constructed wetlands usually provide both shallow and deep areas with aerobic and anaerobic zones, which is suitable for nitrification followed by denitrification of the ammonium-rich leachates. Full-scale treatment systems are influenced by climatic variables that affect the microbial community. Also, the operational strategy can have a considerable impact on both activity and composition of the microorganisms. Many studies have measured inflow/outflow water quality in treatment systems. Such “black box” studies describe the treatment efficiency but add little to an increased understanding of theorganisms performing the treatment or the spatial distribution of their activities and treatment processes.

In this thesis we investigated seasonal and annual changes in potential nitrification and denitrification, and in the corresponding bacterial communities in constructed wetlands treating landfill leachates. Variations in the potential activity in full-scale systems were investigated over several years, using short-term incubation. The composition of the bacterial communities was investigated using a group-specific PCR primer pair targeting the 16S rRNA genes or a primer pair targeting the funtional gene nosZ. The PCR products were analysed by denaturing gradient gelelectrophoresis and subsequent nucleotide sequencing and phylogentic analysis.

A stable ammonia-oxidising bacterial (AOB) community composition and potential ammonia-oxidation (PAO) were detected in the system with a year-round operation. On the other hand, changes in the AOB community composition which followed the operational schedule were detected in the overland flow area (OFA) running seasonally. Furthermore, the influence of operational strategy was indicated by a low PAO in the wastewater overland flow area and compact constructed wetland receiving high hydraulic loads, indicating the value of aeration. Higher PAO was detected in the OFAs where the hydraulic load followed literature guidelines.

All systems supported diverse AOB communities, represented by several Nitrosomonas and Nitrosospira populations. The number of different populations detected in these wetlands was much higher than reported in municipal wastewater treatment plants, and differed from those in a parallel OFA treating municipal wastewater. Furthermore, the large variation in both potential activity and sequences detected in replicate samples suggests that such systems are spatially heterogenic.

Place, publisher, year, edition, pages
Linköping: Linköping University Electronic Press, 2008. 47 p.
Linköping University Medical Dissertations, ISSN 0345-0082 ; 1067
National Category
Medical and Health Sciences
urn:nbn:se:liu:diva-17923 (URN)978-91-7393-872-3 (ISBN)
Public defence
2008-06-04, Aulan, Hälsans hus, Campus US, Linköpings Universitet , Linköping, 14:00 (English)
Available from: 2009-04-24 Created: 2009-04-24 Last updated: 2009-04-24Bibliographically approved

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