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Protein biochips patterned by microcontact printing or by adsorption-soft lithography in two modes
Linköping University, Department of Physics, Chemistry and Biology, Biomolecular and Organic Electronics . Linköping University, The Institute of Technology.
Linköping University, Department of Physics, Chemistry and Biology. Linköping University, The Institute of Technology.
Linköping University, Department of Physics, Chemistry and Biology. Linköping University, The Institute of Technology.
Linköping University, Department of Physics, Chemistry and Biology, Biomolecular and Organic Electronics . Linköping University, The Institute of Technology.
2008 (English)In: BIOINTERPHASES, ISSN 1559-4106 , Vol. 3, no 3, 75-82 p.Article in journal (Refereed) Published
Abstract [en]

Patterning of proteins is critical to protein biochips. Printing of layers of proteins is well established, as is adsorption of proteins to surfaces properly modified with surface chemical functionalities. The authors show that simple methods based on soft lithography stamps can be used to prepare functional antibody chips through both these routes. Both methods incorporate transfer of the stamp material poly (dimethylsiloxane) (PDMS) to the biochip, whether intended or not intended. The results indicate that microcontact printing of proteins always includes PDMS transfer, thereby creating a possibility of unspecific adsorption to a hydrophobic domain.

Place, publisher, year, edition, pages
2008. Vol. 3, no 3, 75-82 p.
National Category
Natural Sciences
Identifiers
URN: urn:nbn:se:liu:diva-17907DOI: 10.1116/1.2988771OAI: oai:DiVA.org:liu-17907DiVA: diva2:212995
Available from: 2009-04-26 Created: 2009-04-24 Last updated: 2010-04-20
In thesis
1. Conjugated Polyelectrolytes in Interactions with Biomolecules for Supramolecular assembly and Sensing
Open this publication in new window or tab >>Conjugated Polyelectrolytes in Interactions with Biomolecules for Supramolecular assembly and Sensing
2010 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Conjugated polyelectrolytes (CP) show interesting electrical and optical properties for organic electronics as well as for life science applications. Their possibilities of supramolecular assembly with nanowire like misfolded proteins, amyloids, as well as synthetic polypeptides or DNA forming conducting or luminescent nano composites is highly interesting as being a truly bottom up approach for fabrication of OLEDs, photovoltaic’s as well as logic devices. The conformation and aggregation dependent luminescence properties from the special class of CPs, Luminescent conjugated polyelectrolytes (LCP), have been utilised and developed as sensors to follow and study biomolecular interactions, DNA hybridisation, protein-protein interactions and staining of living cell cultures and tissue slides. In this thesis we are bringing the evolution a few steps further by applying new types of experimental techniques, such as light scattering and fluorescence correlation spectroscopy, combined with standard techniques as soft lithography and different spectroscopy techniques, to gain better knowledge of the optical behaviour of LCPs and their interactions with biomolecules. We explore the optical properties and vibronic transitions of LCPs; their ability of resonance energy transfer with LCPs indicating super lightning behaviour; the opposite fluorescence shift when interacting with α-helical rich polypeptides compared to earlier reports of interactions upon staining of β-rich amyloids; and the possibility of LCPs to influence protein aggregation as well as the possibility of fabricating biochips based on LCPs and soft lithography. Here we also show fundamental limitations to patterning using macromolecular fluids, of general relevance to soft lithography and nanoimprint lithography with low viscosity polymers.

Place, publisher, year, edition, pages
Linköping: Linköping University Electronic Press, 2010. 60 p.
Series
Linköping Studies in Science and Technology. Dissertations, ISSN 0345-7524 ; 1308
National Category
Natural Sciences
Identifiers
urn:nbn:se:liu:diva-54902 (URN)978-91-7393-408-4 (ISBN)
Public defence
2010-05-14, Planck, Fysikhuset, Campus Valla, Linköpings universitet, Linköping, 14:00 (English)
Opponent
Supervisors
Available from: 2010-04-20 Created: 2010-04-20 Last updated: 2014-04-08Bibliographically approved

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Wigenius, JensFransson, Sophiavon Post, FredrikInganäs , Olle

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Biomolecular and Organic Electronics The Institute of TechnologyDepartment of Physics, Chemistry and Biology
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