Genes encoding tetracycline resistance in a full-scale municipal wastewater treatment plant investigated during one year
(English)Manuscript (Other academic)
Tetracycline-resistant bacteria and genes encoding tetracycline resistance are common in anthropogenic environments. We studied how wastewater treatment affects the prevalence and concentration of two genes that encode resistance to tetracycline: tetA and tetB. Using real-time PCR we analysed wastewater samples collected monthly for one year at eight key-sites in a full-scale municipal wastewater treatment plant (WWTP). We detected tetA and tetB at each sampling site and the concentration of both genes, expressed per wastewater volume or per total-DNA, decreased over the treatment process. The reduction of tetA and tetB was partly the result of the sedimentation process. The ratio of tetA and tetB, respectively, to total DNA was lower in or after the biological processes. Taken together our data show that tetracycline resistance genes occur throughout the WWTP and that the concentrations are reduced under conventional operational strategies. However, it is not possible to conclude the eventual risk for humans with respect to resistance spreading.
tetA, tetB, tetracycline, LUXTM real-time PCR, wastewater treatment plant
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:liu:diva-18296OAI: oai:DiVA.org:liu-18296DiVA: diva2:217653