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Purification of psychoactive biomolecules in plants using size exclusion chromatography
Linköping University, Department of Physics, Chemistry and Biology.
2009 (English)Independent thesis Advanced level (degree of Master (Two Years)), 20 credits / 30 HE creditsStudent thesisAlternative title
Rening av psykoaktiva biomolekyler från växtmaterial genom gelpermeations-/gelfiltreringskromatografi (Swedish)
Abstract [en]

Size exclusion chromatography (SEC) was applied for purification of psychoactive biomolecules from plants. These molecules are in the same molecular weight range, but do not necessarily share other chemical properties, that makes the SEC technique efficient. By applying SEC as a first purification step much of the co-extractives from the plants can easily be removed. Large amounts of target substance can be obtained with little effort if the system is automated. Combining SEC with a second purification step, consisting of normal phase chromatography, provides high purity of the target substance.

Both known and unknown psychoactive biomolecules can easily be purified using the purification method developed in this Master's Thesis. Purifications that previously required long time and much "hands-on" can be completed much faster and with less manual work.

The method developed was tested on cannabis, coffee and 'Spice' with good results.

Place, publisher, year, edition, pages
2009. , 51 p.
Keyword [en]
Size exclusion chromatography, Psychoactive, Drug, Plant, Gel permeation chromatography, Gel filtration chromatography
National Category
Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)
Identifiers
URN: urn:nbn:se:liu:diva-18434ISRN: LITH-IFM-A-EX--09/2105--SEOAI: oai:DiVA.org:liu-18434DiVA: diva2:219596
Presentation
2009-05-25, Linden, Hälsouniversitetet, Linköping, 00:00 (Swedish)
Uppsok
fysik/kemi/matematik
Supervisors
Examiners
Available from: 2009-05-28 Created: 2009-05-26 Last updated: 2009-05-28Bibliographically approved

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Masters Thesis(2502 kB)480 downloads
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Ring, Ludwig
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CiteExportLink to record
Permanent link

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Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • oxford
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf