Purification of psychoactive biomolecules in plants using size exclusion chromatography
Independent thesis Advanced level (degree of Master (Two Years)), 20 credits / 30 HE creditsStudent thesisAlternative title
Rening av psykoaktiva biomolekyler från växtmaterial genom gelpermeations-/gelfiltreringskromatografi (Swedish)
Size exclusion chromatography (SEC) was applied for purification of psychoactive biomolecules from plants. These molecules are in the same molecular weight range, but do not necessarily share other chemical properties, that makes the SEC technique efficient. By applying SEC as a first purification step much of the co-extractives from the plants can easily be removed. Large amounts of target substance can be obtained with little effort if the system is automated. Combining SEC with a second purification step, consisting of normal phase chromatography, provides high purity of the target substance.
Both known and unknown psychoactive biomolecules can easily be purified using the purification method developed in this Master's Thesis. Purifications that previously required long time and much "hands-on" can be completed much faster and with less manual work.
The method developed was tested on cannabis, coffee and 'Spice' with good results.
Place, publisher, year, edition, pages
2009. , 51 p.
Size exclusion chromatography, Psychoactive, Drug, Plant, Gel permeation chromatography, Gel filtration chromatography
Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)
IdentifiersURN: urn:nbn:se:liu:diva-18434ISRN: LITH-IFM-A-EX--09/2105--SEOAI: oai:DiVA.org:liu-18434DiVA: diva2:219596
2009-05-25, Linden, Hälsouniversitetet, Linköping, 00:00 (Swedish)
Steffenrud, SteffenDahlén, JohanCarlsson, Andreas