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A method to quantify mouse coat-color proportions
The University of Arkansas for Medical Sciences.
The University of Arkansas for Medical Sciences.
The University of Arkansas for Medical Sciences.
The University of Arkansas for Medical Sciences.
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2009 (English)In: PLoS ONE, ISSN 1932-6203, Vol. 4, no 4, e5414- p.Article in journal (Refereed) Published
Abstract [en]

Coat-color proportions and patterns in mice are used as assays for many processes such as transgene expression, chimerism, and epigenetics. In many studies, coat-color readouts are estimated from subjective scoring of individual mice. Here we show a method by which mouse coat color is quantified as the proportion of coat shown in one or more digital images. We use the yellow-agouti mouse model of epigenetic variegation to demonstrate this method. We apply this method to live mice using a conventional digital camera for data collection. We use a raster graphics editing program to convert agouti regions of the coat to a standard, uniform, brown color and the yellow regions of the coat to a standard, uniform, yellow color. We use a second program to quantify the proportions of these standard colors. This method provides quantification that relates directly to the visual appearance of the live animal. It also provides an objective analysis with a traceable record, and it should allow for precise comparisons of mouse coats and mouse cohorts within and between studies.

Place, publisher, year, edition, pages
2009. Vol. 4, no 4, e5414- p.
National Category
Engineering and Technology
Identifiers
URN: urn:nbn:se:liu:diva-18755DOI: 10.1371/journal.pone.0005414OAI: oai:DiVA.org:liu-18755DiVA: diva2:221297
Note
Original Publication: S. Ounpraseuth, T.M. Rafferty, R.E. McDonald-Phillips, W.M. Gammill, E.R. Siegel, K.L. Wheeler, Erik Nilsson and C.A. Cooney, A method to quantify mouse coat-color proportions, 2009, PLoS ONE, (4), 4, e5414. http://dx.doi.org/10.1371/journal.pone.0005414 Licensed under Creative Commons Available from: 2009-06-10 Created: 2009-06-03 Last updated: 2010-04-28Bibliographically approved

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Citation style
  • apa
  • harvard1
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  • modern-language-association-8th-edition
  • vancouver
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  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
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  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
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