The comparative analysis of subcellular fractionation methods for revealing of a-actinin 1 and a-actinin 4 in A431 cells
2009 (English)In: Tsitologiya, ISSN 0041-3771, Vol. 51, no 2, 122-129 p.Article in journal (Refereed) Published
a-Actinin 1 and a-actinin 4 belong to a family of actin-binding proteins with shared structural function and regulation of several processes in a cell. Based on previous data on different distribution of these proteins in the nucleus and cytoplasm, we have explored in detail the distribution of a-actinin 1 and a-actinin 4 in subcellular fractions in A431 cells spread on fibronectin. Several methods of subcellular fractionation were used. Complex approach allowed resuming that revealing of a-actinin isoforms in fractions depended on the composition of lysis buffer and preliminary low-temperature freezing of the cells. We have drawn a conclusion that a-actinin 4 can be found in all cytoplasmic and nuclear subfractions, while a-actinin 1 is characterized by cytoplasmic and membrane localization with specificity of its distribution tightly to the nuclear membrane.
Place, publisher, year, edition, pages
St Petersburg, Russian Federation: Sankt-Peterburgskaya Izdatel'skaya Firma Nauka , 2009. Vol. 51, no 2, 122-129 p.
α-actinin 1; α-actinin 4; Subcellular fractionation
IdentifiersURN: urn:nbn:se:liu:diva-18756ScopusID: 2-s2.0-65249092142OAI: oai:DiVA.org:liu-18756DiVA: diva2:221298