Association and insulin regulated translocation of hormone-sensitive lipase with PTRF
2006 (English)In: Biochemical and Biophysical Research Communications - BBRC, ISSN 0006-291X, Vol. 350, no 3, 657-661 p.Article in journal (Refereed) Published
Polymerase I and transcript release factor (PTRF) is in human adipocytes mainly localized at the plasma membrane. This localization was under control of insulin, which translocated PTRF to the cytosol and nucleus, indicating a novel role for PTRF in insulin transcriptional control. In the plasma membrane PTRF was specifically bound to a triacylglycerol-metabolizing subclass of caveolae containing hormone-sensitive lipase (HSL). In response to insulin PTRF was translocated to the cytosol in parallel with HSL. PTRF and HSL were quantitatively immunoprecipitated from the cytosol by antibodies against either PTRF or HSL. The findings indicate also a novel extranuclear function for PTRF in the control of lipolysis.
Place, publisher, year, edition, pages
2006. Vol. 350, no 3, 657-661 p.
Hormone-sensitive lipase, Polymerase I and transcript release factor, Adipocyte, Human, Insulin, Translocation, Protein complex, Caveolae, Lipid metabolism, Transcriptional control
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:liu:diva-19148DOI: 10.1016/j.bbrc.2006.09.094PubMedID: 17026959OAI: oai:DiVA.org:liu-19148DiVA: diva2:223387