Blood compatibility of photografted hydrogel coatings
2010 (English)In: ACTA BIOMATERIALIA, ISSN 1742-7061, Vol. 6, no 7, 2599-2608 p.Article in journal (Other academic) Published
In this work we have evaluated the haemocompatibility of different surface modifications, intended for biomaterials and biosensor applications. Polystyrene slides were coated with thin hydrogel films by self-initiated photografting of four different monomers. The hydrogel surface modifications were thoroughly characterized and tested for their protein resistance and ability to facilitate platelet adhesion and activation of the coagulation system. There was very little protein adsorption from human plasma on the hydrogels formed from poly(ethylene glycol) methacrylate (PEGMA) and 2-hydroxyethyl methacrylate (HEMA). Platelet adhesion tests performed under both static and flow conditions showed that these coatings also demonstrated very high resistance towards platelet adhesion. A small amount of platelets were found to adhere to hydrogels formed from ethylene glycol methyl ether methacrylate (EGMEMA) and 2-carboxyethyl methacrylate (CEA). The polystyrene substrates themselves facilitated large amounts of platelet adhesion under both static and flow conditions. Utilizing a novel setup for imaging of coagulation, it was shown that none of the hydrogel surfaces activated the coagulation system to any great extent. We suggest that this simple fabrication method can be used to produce hydrogel coatings with unusually high blood compatibility, suitable for demanding biomaterials applications.
Place, publisher, year, edition, pages
Elsevier Science B.V. Amsterdam , 2010. Vol. 6, no 7, 2599-2608 p.
Hydrogel; Biomaterial; Protein adsorption; Coagulation; Platelet
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:liu:diva-19175DOI: 10.1016/j.actbio.2009.12.046ISI: 000278868000027OAI: oai:DiVA.org:liu-19175DiVA: diva2:223524