Functionalized zinc oxide nanorod with ionophore-membrane coatingas an intracellular Ca2+ selective sensor
2009 (English)In: Applied Physics Letters, ISSN 0003-6951, E-ISSN 1077-3118, Vol. 95, no 2, 23703- p.Article in journal (Refereed) Published
The tip of a borosilicate glass capillary with functionalized hexagonal ZnO nanorods was used to make a sensitive electrochemical intracellular Ca2+ sensor. To adjust the sensor for Ca2+ measurements with sufficient selectivity and stability, polyvinyl chloride (PVC) membrane containing Ca2+ ionophores were coated on the surface. The membrane covered ZnO nanorods exhibited a Ca2+-dependent electrochemical potential difference versus an Ag/AgCl reference electrode. The potential difference was linear over a large concentration range (100 nM to 10 mM). The measurements of Ca2+ concentrations using our ZnO nanorods sensor in human fat cells or in frog egg cells were consistent with values of Ca2+ concentrations reported in the literature. This nanoelectrode device paves the way to measurements of intracellular biochemical species in specific locations within single living cells.
Place, publisher, year, edition, pages
EBSCO/American Institute of Physics , 2009. Vol. 95, no 2, 23703- p.
IdentifiersURN: urn:nbn:se:liu:diva-19516DOI: 10.1063/1.3176441OAI: oai:DiVA.org:liu-19516DiVA: diva2:225282
On the day of the defence date the status of this article was Submitted.2009-06-252009-06-252014-01-15Bibliographically approved